TNF-alpha ۳۰۸G/A Polymorphism and Serum Level of TNF-α Associated with Covid-۱۹ Severity
- سال انتشار: 1402
- محل انتشار: مجله بین المللی تحقیقات پیشرفته زیست شناختی و زیست پزشکی، دوره: 11، شماره: 4
- کد COI اختصاصی: JR_IJABBR-11-4_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 211
نویسندگان
Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
Kerman University of medical science, Kerman, Iran
Kerman University of medical science, Kerman, Iran
Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
چکیده
Introduction: T-lymphocyte-derived cytokines, including TNF-α, play a critical role in regulating cellular functions, particularly the immune response to pathogenic infections. This study investigated the frequency of TNF-α gene ۳۰۸ promoter alleles and measured the serum cytokine levels in both control and patient groups.Methodology: The study was a case-control study that included ۸۴ COVID-۱۹ positive patients from Kerman's Afzalipur Hospital, Iran, and ۸۴ healthy individuals with negative coronavirus test results as the control group. It was used bioinformatics analysis and the PCR-RFLP technique to determine the TNF promoter region's genotype and the ELISA test to measure the cytokine's serum level. Statistical analysis was conducted using SPSS software (Version-۲۰) with a significance level of P< ۰.۰۵.Results: It was found that the TNF promoter region's ۳۰۸ SNP genotype was associated with TFII-I transcription factor binding predictions. However, there was no significant difference in allele frequency between the control and case groups in the TNF-۳۰۸ promoter (P< ۰.۰۵). Patients with COVID-۱۹ had higher serum levels of TNF-α compared to the control group (P< ۰.۰۵). The study also revealed that elevated levels of TNF-α, LDH, ESR, and CRP in the serum can predict severe outcomes in COVID-۱۹.Conclusion: Although the study did not identify significant differences in TNF-α allele -۳۰۸ frequencies between COVID-۱۹ patients and the control group, the results suggest that TNF-α alleles may impact the infection severity. As a result, measuring serum TNF-α levels and identifying TNF-α alleles in COVID-۱۹ patients could be valuable for predicting disease severity and developing targeted therapies.کلیدواژه ها
Promoter, bioinformatics analysis, PCR-RFLP allele frequency, Immune responseاطلاعات بیشتر در مورد COI
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