Molecular Characterization of Zhumeria majdea Iranian Germplasms Using ISSR Markers
- سال انتشار: 1399
- محل انتشار: مجله بین المللی علوم و فنون باغبانی، دوره: 7، شماره: 4
- کد COI اختصاصی: JR_IJHST-7-4_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 60
نویسندگان
Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, Iran
Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, Iran
Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran
Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran
Hormozgan Department of Environmental laboratory
چکیده
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of ۱۳ geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were collected from the main growing habitats of this spices including Haji Abad, Geno and Bastak regions. The collected leaf samples were subjected to DNA extraction followed by PCR assay, using Inter-Simple Sequence Repeats (ISSR) markers. Twelve markers produced totally ۱۲۱ polymorphic bands and revealed a clear-cut among and within Z. majdea genotypes. The analysis of molecular variance (AMOVA) showed ۸۶% and ۱۴% variations within and among populations, respectively. Cluster analysis divided genotypes into four main groups. The first and second principle coordinates allocated ۲۸.۸۱% and ۱۵.۷۱% of the variations, respectively. In addition to the innate differences of the individuals, the high intra population variation of Z. majdea, can also be explained by differences in the presence or absence of endophytes and differences in the type and genetic pattern of their endophytes.کلیدواژه ها
Cluster Analysis, Genetic variation, ISSR marker, Lamiaceae, Zhumeria majdeaاطلاعات بیشتر در مورد COI
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