Bacterial Ice Nucleation Proteins: Features, Structure, and Applications
- سال انتشار: 1402
- محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 10، شماره: 3
- کد COI اختصاصی: JR_JABR-10-3_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 69
نویسندگان
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده
Some bacteria commonly found on plants can catalyze the freezing of water at a higher temperature than others, at or near ۰ °C. The freezing point of pure water is about -۴۰ °C and is initiated by creating ice nucleations. However, when ice nucleation proteins (INPs) are present, ice nucleations form at temperatures close to or above ۰ °C. INPs are often attached to the outer membrane by a phosphatidylinositol anchor and are sometimes secreted extracellularly. The monomers of INPs in Pseudomonas syringae are ۱۲۰ to ۱۸۰ kDa. INP has three domains, and the central domain is highly repetitive. The central domain consists of the consensus sequence of eight amino acid repeats. Eight amino acid repeats create a ۱۶-residue fragment, and three ۱۶-residue fragments form the ۴۸-residue fragment. Studies have shown that INPs may have a β-helical fold and interact with water through the repetitive motif. Most ice nucleation bacteria are gram-negative, including P. syringae, Pseudomonas viridiflava, Pseudomonas fluorescens, Xanthomonas compestris, Erwinia ananas, and Erwinia herbicola. For optimum protein activity, the presence of the complete bacterial cell is essential. INPs are influential in different aspects, including snowmaking, agriculture, freeze-concentration in the food industry, signal transduction, atmospheric applications as cloud condensation nuclei, and surface display (expression of a foreign protein on the cell surface for biotechnological purposes). This study provides a brief overview of ice nucleation proteins and their applications since ice nucleation is an important phenomenon that affects various aspects, from climate to biological systems.کلیدواژه ها
Ice-Nucleating Proteins, Supercooling, Pseudomonas syringae, Silver Iodide, Snow-Makingاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.