Establishment and Characterization of Melanocyte Stem Cells from Hair Follicle Bulge of Vitiligo Patients

  • سال انتشار: 1402
  • محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 10، شماره: 3
  • کد COI اختصاصی: JR_JABR-10-3_004
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 55
دانلود فایل این مقاله

نویسندگان

Vathsalya Manchi

Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Shricharith Shetty

Department of Dermatology, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Nikhil Shetty

Department of Plastic Surgery, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Shama Rao

Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Veena Shetty

Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Sunil Kumar Yeshwanth

Department of Pathology, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

Basavarajappa Mohana Kumar

Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte-۵۷۵۰۱۸, Mangaluru, India

چکیده

Introduction: Melanocyte stem cells (MelSCs) might offer potential candidates for cell-based therapies against pigmentary disorders, such as vitiligo. However, the properties of MelSCs and their involvement in disease pathology are yet to be explored. In this study, MelSCs derived from the lesional (V-MelSCs) and unaffected regions of vitiligo patients (NV-MelSCs) were established, and compared their cellular, biological and molecular characteristics to that of MelSCs isolated from the skin of normal, unaffected individuals as control (C-MelSCs).Materials and Methods: MelSCs were isolated and expanded in vitro by explant culture. Growth kinetics, metabolic activity, cell viability, colony-forming ability, senescence activity, and cell cycle status of MelSCs were analyzed. Later, MelSCs were evaluated for the expression of marker genes, such as CD۳۴, Pax۳, Sox۱۰, sFRP۴, MITF, TYRP۱, DCT, TYR, TGFα, and c-KIT.Results: MelSCs derived from vitiligo patients had lower proliferation and metabolic activity despite showing unaltered morphological features. Significantly (P< ۰.۰۵) decreased number of viable cells was observed in C-MelSCs and V-MelSCs in passage ۳ (P۳) compared to P۱. The colonyforming assay revealed homogeneous distribution, but slightly larger and dense colonies in C-MelSCs compared to NV-MelSCs and V-MelSCs. All MelSCs showed a higher percentage in the G۰/G۱ phase of the cell cycle with low senescence activity. Finally, MelSCs from the skin of vitiligo exhibited lower expression levels of selected marker genes implicated in melanocyte development.Conclusions: MelSCs residing in hair follicle bulge of vitiligo patients possess marginally altered cellular, potency and biological characteristics. These findings underscore the challenges in establishing repigmentation therapy for vitiligo using autologous transplantation of MelSCs.

کلیدواژه ها

Hair Follicle Bulge, Melanocyte Stem Cells, Vitiligo, In Vitro, Comparative Characterization

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.