Naif Mohammed Alotaibi - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, Jeddah, Saudi Arabia
Saleh M. Al-Maaqar - Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box ۸۰۲۰۳, Jeddah ۲۱۵۸۹, Saudi Arabia
Amna A. Saddiq - College of Sciences, Department of Biology, University of Jeddah, Jeddah, Saudi Arabia
Saber Abdelkader Saidi - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, Jeddah, Saudi Arabia
Mohamed Mohamed Roushdy - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, Jeddah, Saudi Arabia
Turki Alsheikh - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, Jeddah, Saudi Arabia
Naser Alkenani - Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box ۸۰۲۰۳, Jeddah ۲۱۵۸۹, Saudi Arabia
Mohammad Reza Mohammadi - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Since the beginning, mosquito-borne diseases like dengue fever, encephalitis, yellow fever, malaria, and filariasis have been caused by numerous medically significant pathogens and parasites, including viruses, bacteria, and protozoans. This indicates the necessity for the ongoing creation of new and effective mosquito-borne disease control strategies in Saudi Arabia and internationally. This investigation has tried to assess the potential larvicidal capacity of local bacteria isolated from the soil of the Rahat region of Makkah, Saudi Arabia for the bio-control of Aedes aegypti larvae, a main cause of dengue. The bacteria were identified using morphological and molecular characteristics. Bioassays were used to determine the pathogenicity of various strains against A. aegypti larvae. A total of ۶۶ different bacteria were isolated. Overall, four (۶.۰۶%) of the ۶۶ bacteria caused mortality in the A. aegypti larvae, and only two (Brevibacillus centrosporus, and Cytobacillus firmus) caused ۱۰۰% mortality in ۲۴ h. After ۴۸ h, two isolates (Escherichia fergusonii۱ and E. fergusonii ۲) caused mortality of over ۷۰%. The outcomes of this investigation exhibited that local isolates of bacteria in the soils of the Rahat region of Makkah, Saudi Arabia, have larvicidal ability. These bacteria have shown larvicidal effects on the larvae of A. aegypti. In conclusion, further studies are required to evaluate other mechanisms that contribute to the production of larvicidal toxins in these bacteria.

Larvicidal potential, Aedes aegypti, bio-control, Brevibacillus centrosporus, Cytobacillus firmus, Escherichia fergusonii