Crocin Protects Against Beta-Amyloid Peptide-Induced Cell Apoptosis in PC۱۲ Cells Via the PI۳ K Pathway

سال انتشار: 1402
محل انتشار: چهاردهمین کنفرانس بین المللی بهداشت، درمان و ارتقای سلامت
کد COI اختصاصی: HWCONF14_083
زبان مقاله: انگلیسی
تعداد مشاهده: 258

نویسندگان

Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Elham Hadipour

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Background: Crocin is a known compound with the antioxidant and anti-inflammatory properties which may help to reduce the progression of neurological disorders. In this study, we aimed to investigate the protective effects of crocin on beta-amyloid peptide Aβ (۱-۴۰) and hydrogen peroxide (H۲O۲) induced neurotoxicity in PC۱۲ cells.Methods: PC۱۲ cells were pretreated with crocin and donepezil (۵ and ۱۰ μM) for ۲ h and then treated with Aβ (۱-۴۰) (۲۵ μM) for ۲۴ h. In parallel, after pretreatment with crocin (۵ and ۱۰ μM) and donepezil (۵ and ۱۰ μM) for ۲۴ h, cells were treated with H۲O۲ (۸۰۰ μM) for ۴ h. Finally, the cell viability and intracellular reactive oxygen species (ROS) generation were evaluated using AlamarBlue® and ۲', ۷'-dichlorodihydrofluorescein diacetate (DCFH-DA), respectively. The western blot test was done to compare the protein level of phospho SAPK/JNK, SAPK/JNK, PI۳ Kinase P۸۵, Phospho-PI۳ Kinase P۸۵, caspase-۳ and cytochrome c) cyt c).Results: Crocin and donepezil could significantly decrease the Aβ toxicity and ROS level. While treatment with Aβ increased Cyt c release from mitochondria to cytosol, cleaved form of caspase-۳ (۱۷ kDa) and activated form of SAPK/JNK p۴۴/۴ decreased the activated form of PI۳ Kinase P۸۵ protein, indicating that crocin could significantly block the apoptosis initiated with Aβ.Conclusion: According to the results, crocin could be a promising candidate for further evaluations against the development of Alzheimer's disease through mitogen-activated protein kinases (MAPK) and the phosphatidylinositol ۳-kinase (PI۳K)/protein kinase B (AKT) signaling (PI۳ K/AKT) pathways.

کلیدواژه ها

Alzheimer's disease, beta-amyloid, crocin, hydrogen peroxide, donepezil, oxidative stress

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