Essential Oil Components and Antitrichomonal Effects of Piper nigrum L.
- سال انتشار: 1402
- محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 30، شماره: 4
- کد COI اختصاصی: JR_JKMU-30-4_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 212
نویسندگان
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Medical Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
چکیده
Background: Trichomoniasis, caused by Trichomonas vaginalis protozoan, is the most common nonviral sexually transmitted infections worldwide. Although metronidazole and tinidazole are the only approved drugs for treatment, drug-resistant cases of infection are on the rise. The aim of the study was the evaluation of antitrichomonal potential of Piper nigrum and limonene. The phytochemical profile of P. nigrum oil was also investigated. Methods: The parasites were treated in vitro with essential oil and different extracts of P. nigrum seed and limonene using microtiter plate method. The oil analysis of P. nigrum was also evaluated by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. Furthermore, the cytotoxicity assay of P. nigrum oil and limonene were screened on Vero cell line by MTT method.Results: The tested P. nigrum fractions were able to kill ۱۰۰% of Trichomonas trophozoites at MLC concentration and reduce the trophozoite viability at sub-MLC and lower concentrations. After ۴۸ hours exposure, the most potent fraction was the n-hexane extract with MLC of ۷۸ µg/ml and followed by the essential oil and methanol extract with MLC of ۱۵۶ µg/ml, limonene (MLC=۱۲۵۰ µg/ml), and then, aqueous extract with MLC value of ۲۵ mg/ml. Also according to cytotoxicity assay, P. nigrum oil was less toxic to Vero cell than limonene, with a selectivity index of ۱۳.۲ and ۲.۰۴, respectively. Conclusion: This study clearly demonstrated the trichomonacidal potential of P. nigrum. Thus, P. nigrum fractions can be considered promising antiprotozoal agents and the basis for further development to discover new phytochemicals compounds.کلیدواژه ها
Essential oil, Extract, Limonene, Piper nigrum, Trichomonas vaginalisاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.