Using Micro-Extraction on a Screw for Determination of Trace Amounts of Cationic Dyes by HPLC-UV/Vis

  • سال انتشار: 1400
  • محل انتشار: دهمین سمینارملی شیمی و محیط زیست ایران
  • کد COI اختصاصی: NSCEI10_064
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 125
دانلود فایل این مقاله

نویسندگان

P Osouli

Department of Chemistry, Faculty of Science, Tarbiat Modares University, P.O. Box ۱۴۱۱۵-۱۷۵, Tehran,Iran

Y Yamini

Department of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box ۴۵۱۹۵-۱۱۵۹, Zanjan, Iran

N Nikfarjam

Department of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box ۴۵۱۹۵-۱۱۵۹, Zanjan, Iran

S Nasrollahi

Department of Chemistry, Faculty of Science, Tarbiat Modares University, P.O. Box ۱۴۱۱۵-۱۷۵, Tehran, Iran

M Tabibpur

Chemistry & Chemical Engineering Research Center of Iran, P.O. Box ۱۴۳۳۴-۱۸۶, Tehran, Iran

چکیده

Nowadays, among all pollutants, cationic dyes are one of the biggest concerns incontemporary society. These dyes pose a serious threat to human health and the environment dueto their toxic, non-biodegradable, and even carcinogenic effects. Therefore, it is important todevelop sensitive techniques for determining these colors in real samples [۱].In general, sample preparation is an important step in separation techniques and accuratedetermination of the desired analyte from complex matrices [۲]. Among the sample preparationmethods, solid phase micro-extraction (SPME) is more popular. SPME is found useful for microextractiondue to its small geometry and simple operating procedures, simplicity, rapidity, accurateanalysis and low consumption of organic solvent [۳].In the present work, solid phase micro-extraction on a screw (MES) which is a miniaturized formof in-tube SPME without back pressure, was developed to extract cationic dyes (malachite green,methylene blue and rhodamine B) in water samples. The screw was coated with a functionalizedco-polymer as an absorbent. The extracted dyes on the screw was eluted by a suitable eluent.Separation and determination of the dyes was performed by high performance liquidchromatography equipped with a UV detection (HPLC-UV). Hence, MES is prepared byelectrophoretic coating sulfonated poly (styrene-co-divinylebenzene) on surface of a screw. Thenit was placed inside a silicon tube to create a channel to pass sample solution through it. Effectiveparameters on extraction efficiency of analytes were studied and optimized. Under optimumconditions, the limit of detection was ۰.۱۵ μg L-۱ and calibration curve was liner in the range of۰.۵-۲۵۰ μg L-۱ with a correlation coefficient greater than ۰.۹۸ for all studied cationic dyes. Theinter-day and intra-day precision (n=۵) were ۲.۸-۷% and ۷-۹.۵%, respectively. The proposedmethod is evaluated as a simple and repeatable method with acceptable relative recoveries (۸۲-۱۰۳%) for determination of cationic dyes in real water samples.

کلیدواژه ها

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.