Effect of the complex of β-glucan and Zataria multiflora on oxidative burst of neutrophils in BALB/c mice
- سال انتشار: 1402
- محل انتشار: سومین کنفرانس بین المللی فناوری های نوین در علوم
- کد COI اختصاصی: CMTS03_015
- زبان مقاله: انگلیسی
- تعداد مشاهده: 131
نویسندگان
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده
Natural products such as β-glucan and herbal essences have been found to enhance immune functions. The aims of this study were to purify β-glucan from the cell wall of Saccharomyces cerevisiae and to investigate its conjugate effect with Zataria multiflora essence on oxidative burst in BALB/c mice. After preparing the cell walls from the yeast, β-glucan was extracted from wall by alkaline-acid treatment (glucan-S۱). This preparation contained protein and mannan, which were removed by DEAE sephacel chromatography (glucan-S۲) and con-A sepharose chromatography (glucan-S۳), respectively. Glucan-S۳, Z. multiflora essence, and their conjugate were injected into BALB/c mice intraperitoneally. Blood was collected at days ۴ and ۷ after injection and oxidative burst was assayed by chemiluminescence method. The results showed that intraperitoneally administration of glucan-S۳, Z. multiflora essence and their conjugate significantly increased oxidative burst of neutrophils both at days ۴ and ۷ when compared to control group (P< ۰.۰۵). These findings suggest that the complex of β-glucan and Z. multiflora essence can be used as adjuvant agents to stimulate immune functions in immunocompromised subjects.کلیدواژه ها
Saccharomyces cerevisiae, β-glucan, Zataria multiflora essence, oxidative burst.اطلاعات بیشتر در مورد COI
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