Studies on Genotoxic Effects of Mobile Phone Radiation on A۳۷۵ Cells

  • سال انتشار: 1398
  • محل انتشار: مجله فیزیک پزشکی ایران، دوره: 16، شماره: 1
  • کد COI اختصاصی: JR_IJMP-16-1_011
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 96
دانلود فایل این مقاله

نویسندگان

Debashri Manna

Department of Biochemistry & Biophysics, University of Kalyani, Kalyani, Nadia, India

Sagar Sanyal

Dept. of Physiology, West Bengal University of Animal and Fishery Sciences, ۳۷, K. B. Sarani, Kolkata– ۷۰۰۰۳۷, West Bengal, India

Rita Ghosh

Department of Biochemistry & Biophysics, University of Kalyani, Kalyani, Nadia, India

چکیده

Introduction: Radiation from cell phones has been associated with an increased risk of cancer. The literature has reported evidence of certain biological effects resulting from exposure to various wavelengths, doses, and intensities of radiofrequency radiation. The present study aimed to evaluate the possible adverse effects of radiation from a GSM mobile phone operating at ۹۰۰ MHz on human melanoma A۳۷۵ cells. Material and Methods: Cellular morphology was observed under an inverted phase contrast microscope. Cell viability was determined through trypan blue dye exclusion and clonogenic assay. Moreover, flow cytometry was applied to detect DNA damage, cell cycle arrest, and reactive oxygen species (ROS) production. Cellular reduced glutathione (GSH) content was estimated by measuring the total soluble thiol. In addition, the physico-chemical changes were assessed using spectrophotometer and viscometer. Results: This study revealed that there was no change in cellular morphology and necrotic cell killing; although a small effect was observed on delayed cell death. Depletion in GSH content was noted, but ROS generation was not significantly different from that of the control group. No DNA damage was found during such exposure and there was no alteration in cell cycle distribution. In vitro evaluation of radiation effect on calf thymus DNA showed a slight perturbation in absorption spectra that was completely reversible with time. On the other hand, viscometric analysis showed no changes. Conclusion: From the findings, it can be concluded that this range of mobile phone radiation for ۶۰ min of continuous exposure has no genotoxic impact on A۳۷۵ cells.

کلیدواژه ها

Cell Cycle Arrest, DNA damage, Electromagnetic Radiation, Reduced Glutathione, Reactive Oxygen Species, Viability

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.