Evaluation of the expression profile of mRNAs and lncRNAs in cumulus cells associated with polycystic ovary syndrome and pregnancy
- سال انتشار: 1402
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 10
- کد COI اختصاصی: JR_IJBMS-26-10_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 206
نویسندگان
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
چکیده
Objective(s): Polycystic ovary syndrome (PCOS), the primary cause of anovulatory infertility in women, may change the gene expression profile of cumulus cells. In human ART (assisted reproductive technology), gene expression profiling in cumulus cells, a non-invasive method, may be used to identify the most competent oocytes. We aim to identify key genes according to the network-based data and assess the suitability of these genes as markers to predict oocyte competence and PCOS diagnosis.Materials and Methods: The GSE۳۴۵۲۶ microarray dataset was obtained from the Gene Expression Omnibus (GEO) database. The function and pathway enrichment analysis for DEGs were analyzed. A protein-protein interaction (PPI) network analysis and candidate gene screening were conducted. A two-layer network consisting of mRNA and lncRNA was constructed. Expression levels of hub genes were verified using quantitative RT-PCR (qRT-PCR).Results: A total of ۲۷۲۱ DEGs were retained. The PPI network of selected genes associated with the biological process of “cell communication” was analyzed, and the first ۱۰ key genes were determined by degree. Additionally, ۲ hub genes and ۲ hub lncRNAs, including STAT۳, RHOA, GAS۵, and LINC۰۱۱۱۶, were selected from the lncRNA-mRNA network. Finally, expression levels of STAT۳, RHOA, GAS۵, and LINC۰۱۱۱۶ were significantly increased in the cumulus cells of PCOS patients compared to the control group (P< ۰.۰۵). However, there was no significant difference in expression between the pregnant and non-pregnant groups.Conclusion: STAT۳, RHOA, GAS۵, and LINC۰۱۱۱۶ may serve as possible diagnostic markers for PCOS. However, further studies on a larger population are needed to validate this finding.کلیدواژه ها
Cumulus cells, Infertility, Long non-coding RNA, Polycystic ovary syndrome, Systems Biologyاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.