H Nasrollahzadeh - Department of Engineering, Shahroud Branch,Islamic Azad University,Shahroud, Iran
M Jahanshahi - Nanotechnology Reasearch Institute, Faculty of Chemical Engineering, Babol University of Technology
S Manafi - Department of Engineering, Shahroud Branch,Islamic Azad University,Shahroud, Iran
M Nasrollahzadeh - Department of Chemistry, Faculty of Science,Semnan University,Semnan,Iran

While nanobioteccnology is developing worldwide for establishing sustainable life, using nanoparticle is also helpful for biological applications. Papain is a highly active plant proteaseobtained from Carica papaya Linn. The physicochemical properties of papain recommend that it could be appropriate for novel food and drug delivery system. During this analysis, fabrication of papain nanoparticles by simple coacervation method was carried out. Following the desolvation of papain with acetone, the made nanoparticles were stabilized and cross linked by addition 200 μl of 25% glutaraldehyde. The nanoparticle sample was purified with 15000 rpm centrifuge for 20 min, then the supernatant was dialyzed. Moreover, fabricated nanoparticles were analyzed by dynamic light scattering (DLS) as well as atomic force microscopy (AFM). The mean particle size of the produced nanoparticles achieved was 107 nm

Papain, Nanoparticles, Simple Coacervation Method, Dynamic Light Scattering