Reinforcing of decellularized human amniotic membrane laminates for skin transplantation

  • سال انتشار: 1401
  • محل انتشار: نهمین کنگره سالانه زخم و ترمیم بافت
  • کد COI اختصاصی: WTRMED09_060
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 168
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نویسندگان

Saeid Kargozar

Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad

Seyed Javad Hoseini

Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Alireza Ebrahimzadeh-Bideskan

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Simin Nazarnezhad

Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad

Seyede Atefe Hosseini

Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Human amniotic membrane (HAM) has been widely used as a biodegradable and bioactive matrix for simulating the natural microcellular environment and tissue regeneration. This study fabricated a multilayered composite of decellularized HAM (d-HAM) for skin transplantation. The multilayered d-HAM was created by repeatedly depositing wet layers of d-HAM and dehydrating them, followed by chemical cross-linking to tighten integration at the layer interfaces and within the layers. Our findings revealed improved physical properties of the laminates by increasing mechanical strength. The microstructure analysis of laminate indicated the tightened cross-linking of layers and flattened structure. Furthermore, the laminates had sufficient biocompatibility and cell migratory impacts in vitro that are crucial factors in wound healing process. conclusion: The current findings are a promising advance in the engineering of novel biomaterials and the alteration of existing tissues for medical use.

کلیدواژه ها

Human amniotic membrane, laminate, skin tissue regeneration, decellularization

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