Farzaneh Tafvizi - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
Khatereh Baghdadi - Department of Biology, Islamic Azad University, Arak Branch, Arak, Iran
nasim Hayati Roodbari - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Background and Aim: Infertility refer to an inability to conceive (irrespective of causes) after having regular unprotected sex. Men are responsible for infertility in ۵۰% of infertile spouses. Numerous factors contribute to male infertility including genital infections that may appear following microbial, fungal, and viral infections. The aim of the present study was detection of Human cytomegalovirus (HCMV) infection in the semen of infertile men and evaluation of its relation with sperm parameters such as motility, count and morphology. Materials and Methods: In this study, ۱۰۰ semen samples of fertile men (as a control group) and ۱۰۰ semen samples of infertile men were collected from Infertility Center of Qom Jahad Daneshgahi, Iran. The semen samples were analyzed according to World Health Organization’s standard methods (WHO). After DNA extraction, cytomegalovirus was detected using Nested polymerase chain reaction technique. The results were analyzed using SPSS (version ۱۹) software (Significance level of P<۰.۰۵). Statistical assessments were conducted using descriptive statistics including the Chi-square test and t test. The difference was considered significant at the P<۰.۰۵ level. Results: The analyzed sperm parameters of ۱۰۰ infertile samples showed that ۵۶%, ۷۰%, and ۸۲% of the samples had problems, respectively in terms of count, motility, and morphology. However, ۶۰% of the infertile samples had problems in all three parameters. Human cytomegalovirus DNA was detected in six and four samples of infertile and fertile men respectively. Conclusions: According to statistical analysis, no significant correlation was obtained between the cytomegalovirus infection and male infertility. Although the achieved results may vary with changes in population size.

Male infertility, Human cytomegalovirus, sperm quality, Nested-PCR, ناباروری مردان, ویروس سایتومگال, کیفیت اسپرم, Nested-PCR