Detection of Shigella dysenteriae STX۱ gene from Mazandaran province clinical samples by PCR-ELISA method
- سال انتشار: 1395
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 10، شماره: 5
- کد COI اختصاصی: JR_IJMM-10-5_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 148
نویسندگان
Department of Biology, Islamic Azad University Amol branch, Amol, Iran
Department of Biology, Islamic Azad University Amol branch, Amol, Iran
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Immunology, Islamic Azad University Babol branch, Babol, Iran
چکیده
Background:Among enterobacteriaceae bacteria, Shigella dysenteriae produce a shiga protein toxin, and have cytotoxicity, enterotoxin and neurotoxin activity. The toxin causes diseases such as diarrhea, gastroenteritis, intravascular coagulation disorder. Today diarrhea is the most important challenge for the human health. The classic and conventional microbiological detection methods are sensitive and specificity, there is limitation. This study was designed to identify shigella dysenteriae in shortest time and the amount of toxin Stx۱ with high sensitivity and specificity by using PCR-ELISA method. Method and material: The Stx۱ sequence (۴۹۰bp) as a target gene was amplified by Dig-dUTP labeled, then product was coating on microplate and by using anti antibody digoxigenin conjugated detection was done. Also the specificity and sensitivity of method with clinical specimens examined. Results: Sensitivity detection of bacteria in the sample using genomic DNA for Shigella dysenteriae was ۱/۵۶pg and specificity of technique didn’t showed acceptable OD in other species of enterobacteriaceae family. ELISA for genomic DNA Up to dilution ۰/۱۵۶ pg had significant absorption. As well as from ۷۰ clinical samples which was analyzed, ۳ samples contained stx۱ gene. Conclusion: Efficiency PCR-ELISA techniques showed that it was simple, faster, high specific and sensitive methods. This technique is more suitable than culture and PCR method also it was easily can applied in each medical laboratory.کلیدواژه ها
Shigella dysenteriae, Shiga toxin, Digoxigenin, PCR-ELISA, شیگلا دیسانتریه, شیگاتوکسین, دیگوکسی ژنین, PCR-ELISAاطلاعات بیشتر در مورد COI
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کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.