O-serogrouping of Escherichia coli strains isolated from patients with Urinary tract infection by using PCR method
- سال انتشار: 1395
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 10، شماره: 6
- کد COI اختصاصی: JR_IJMM-10-6_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 189
نویسندگان
Department of Cell and Molecular Biology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
Department of Medical Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Background and Aim: Urinary tract infection (UTI) is one of the most common infections, and Escherichia coli is so far the most common causative agent of this disease. The O antigen, consisting of many repeats of an oligosaccharide unit, is part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria. The variability of the O antigen provides the major basis for serological schemes of Gram-negative bacteria. The aim of this study was to investigate the prevalence of O-serogroups among E. coli isolated from patients with UTI. Materials and Methods: During three months, ۲۱۰ patients with urinary tract infection, referred to three hospitals of Tehran, Sanandaj and Boroujerd. E. coli isolates were identified using standard methods then serogrouping was performed by using PCR method. Results: A total of ۱۵۰ E. coli strains were isolated from the urine samples. The most common types of O antigens were O۱ (۱۳.۳۳%), O۱۶ (۸%), O۸ (۵.۳۳%) and O۲۱ (۴%). There was no positive isolate for O۲۲ and O۸۳ genes. Conclusions: The present study revealed that serogroup O۱ was isolated with maximum frequency followed by O۱۶, O۸ and O۲۱, respectively. In UPEC, the O-serogroups can be related to the virulence factor profile of each strain. Further studies from other parts of Iran and on other serogroup are recommended.کلیدواژه ها
Escherichia coli, Urinary tract infection, O-Antigen, اشریشیاکلی, عفونت دستگاه ادراری, آنتی ژن Oاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.