Prediction of antigenic sites on ALS۱ and HWP۱ protein sequences in vaginal isolated C. albicans of using bioinformatics analysis
- سال انتشار: 1394
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 9، شماره: 1
- کد COI اختصاصی: JR_IJMM-9-1_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 174
نویسندگان
Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.
چکیده
Background and Aim: The ability to predict antigenic sites on proteins is of major importance for medication. The aim of this study was to predict the antigenic sites on Agglutin in Like Sequence (ALS۱) and Hyphal Wall Protein Sequences (HWP۱) in Candida albicans isolated of vaginal infections using Physico-Chemical Profiles server. Materials and Methods: ۷ isolates were obtained from women with vaginal infection which were collected from various medical centers of Tehran in ۲۰۱۱ and ۲۰۱۲. At the first,DNA was extracted by Phenol-Chloroform method. Multiplex PCR was performed by using specific primers. In order to do bioinformatic studies, the genes were sequenced and then translated. Antigenic sites of protein sequences were identified by Physico-Chemical Profiles program. Results: The results showed that the presence of two genes als۱ and hwp۱ in isolates. In ALS۱ and HWP۱, respectively ۲ and ۱ antigenic site with the most antigenicity were identified. Conclusions: According to previous studies, Serine and Threonine phosphorylation is an important mechanism in pathogenesis of ALS۱ and HWP۱ proteins. Results in this study showed that serine and threonine are the most amino acids in the antigenic sites with high antigenicity property.کلیدواژه ها
Candida albicans, Biofilm, Vaginal infection, Bioinformatics, کاندیدا آلبیکنس, بیوفیلم, عفونت واژینال, بیوانفورماتیکاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.