Transforming Growth Factor-β۱ Preserves Bovine Nasal Cartilage against Degradation Induced by Interleukin-۱α in Explant Culture
- سال انتشار: 1396
- محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 4، شماره: 2
- کد COI اختصاصی: JR_JIML-4-2_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 140
نویسندگان
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Microbiology Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
چکیده
Background and Aims: Chondrocytes and their differentiation play a central role in joint diseases. Effect of the transforming growth factor (TGF)-β۱ on chondrocyte characteristics and differentiation is not clearly understood. This study was undertaken to investigate the effects of TGF-β۱ on tissue characteristics and morphology of chondrocytes against degradation induced by interleukin (IL)-lα in bovine nasal cartilage (BNC) explant culture. Materials and Methods: BNC explants were cultured in DMEM and samples were divided into four groups. In group A (control); samples were only cultured in DMEM and group B; was treated with IL-۱α (۱۰ ng/ml), group C; treated with TGF-β۱ (۱۰ ng/ml) and group D; treated with IL-۱α (۱۰ ng/ml) + TGF-β۱ (۱۰ ng/ml) for ۱۴ days. At days ۳, ۷ and ۱۴ the media were collected and replaced with fresh media. Then, samples were harvested on days ۳, ۷ and ۱۴ and chondrocyte morphology was assessed by invert microscopy. We used Masson's Trichrome stain to visualize collagen distribution and synthesis, and Safranin O and Alician blue to highlight the proteoglycan content. Results: In the presence of IL-lα, most of the chondrocytes were transformed into fibroblast-like morphology with pyknotic nuclei at day ۱۴ and proteoglycan and collagen in extracellular matrix (ECM) were destructed at this time. In the presence of TGF-β۱, chondrocytes preserved their ordinary normal features. Also, TGF-β۱ inhibited collagen and proteoglycan destruction and cartilage ECM showed normal characteristics. Conclusions: IL-۱α induced significant morphological changes in chondrocytes and increased the destruction of ECM. TGF-β۱ could strongly preserves cartilage from IL-۱α degradation effects.کلیدواژه ها
Bovine nasal cartilage, Chondrocyte, IL-۱α, TGF-β, کندروسیت, اینترلوکین ۱ آلفا, فاکتور رشد تغییر دهنده β, غضروف بینی گاواطلاعات بیشتر در مورد COI
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