Study of different doses of zinc oxide nanoparticles by intraperitoneal and gavage methods on testicular tissue in Wistar rats: An experimental study
- سال انتشار: 1402
- محل انتشار: مجله طب تولید مثل ایران، دوره: 21، شماره: 6
- کد COI اختصاصی: JR_IJRM-21-6_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 90
نویسندگان
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran.
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran.
Department of Animal Science Chaloos Branch, Islamic Azad University, Babol, Iran
چکیده
Background: Zinc oxide nanoparticles (ZnO-NPS) are widely used in human life; however, they do have side effects on human health. Objective: This study aimed to evaluate the different doses of ZnO-NPS on testicular tissue. Materials and Methods: ۳۵ male Wistar rats (۱۰-۱۲ wk, ۲۲۰ ± ۲۰ gr) were divided into ۷ groups of ۵, including the control group (gavaged distilled water daily), sham group (received intraperitoneal doses of distilled water twice a week). The group received intraperitoneal ZnO-NPS (۲۵, ۵۰, and ۱۰۰ mg/kg body weight, twice a week), and gavage (۱۵۰ and ۲۰۰ mg/kg body weight daily). All stages of the test were performed in ۴ wk then serum testosterone and tissue malondialdehyde, and ferric reducing antioxidant power levels were measured, also testes histopathological evaluation was performed. Results: Our results showed that a reduced cell population of spermatozoa was observed in the group that received ۲۵ mg/kg ZnO-NPS, while a reduced cell population of spermatozoa, edema, hyperemia, and vacuolar degeneration were observed in the group that received ۵۰ and ۱۰۰ mg/kg ZnO-NPS. The maximum amount of lesions were observed in the dose of ۲۰۰ mg/kg. The highest amount of ferric reducing antioxidant power and testosterone levels were observed in the control group. Also, a ۱۰۰ mg/kg intraperitoneal dose of ZnO-NPS and ۱۵۰ mg/kg oral dose of ZnO-NPS were suitable doses to create a model of male genital lesions. Conclusion: Nanoparticles are harmful factors for the reproductive system and consequently affect infertility, which requires the toxicity of the concentration of these nanoparticles to be evaluated and controlled.کلیدواژه ها
Zinc oxide, Testis, Nanoparticle, Oxidative stress, Testosterone., اکسید روی, بیضه, نانوذرات, استرس اکسیداتیو, تستوسترون.اطلاعات بیشتر در مورد COI
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