The Protective Effect of Curcumin on the Proliferation and Colonization of Spermatogonial ‎Stem Cells ‎in Gamma-Irradiated Rats

  • سال انتشار: 1401
  • محل انتشار: مجله علوم پیشرفته زیست پزشکی، دوره: 12، شماره: 2
  • کد COI اختصاصی: JR_JABS-12-2_009
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 131
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نویسندگان

ساره آزرمی

Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

خسرو حسینی پژوه

Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

پرویز تاجیک

Department of Theriogenology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

مرضیه لشکری

Department of Radiotherapy, Tehran University of Medical Sciences, Tehran, Iran

چکیده

Background & Objective: One of the side effects of radiotherapy can be damage to spermatogonial stem cells that ‎may lead to spermatogenesis disorders and sterility. Protective effects of curcumin on normal cells ‎against radiotherapy side effects have already been shown. In the current study, the protective effects of curcumin ‎on the spermatogonial stem cells against gamma radiation were ‎evaluated.‎ Materials & Methods: This study was done on ۵۰ adult rats in ۱۰ experimental groups. Four groups were ‎injected ۰, ۲۵, ۵۰, or ۱۰۰mg/kg of curcumin in ۱ml olive oil for ۱۵ days intraperitoneally, then exposed to radiation ‎at ۲ Gy on the next day. Also, four groups were treated like above but without radiation; and two groups as control with and without radiation.  The day after radiation, all of the rats were euthanatized, their testes were removed, and they underwent enzymatic digestion to co-culture spermatogonial stem cells. After ۱۲ days, the colonization of spermatogonial stem cells was assessed. ‎ Results: There was a significant decrease in the colonization of spermatogonial stem cell proliferation ‎in groups that had taken radiation but not curcumin. There was a significant increase ‎ in the colonization of spermatogonial stem cells in the group which had taken radiation whit maximum curcumin compared with the other irradiated groups and ‎was similar to non-irradiated control animals. Colonization of spermatogonial stem cells in non-irradiated animals treated with curcumin had increased compared with control groups.‎ Conclusion: Injection of curcumin can protect spermatogonial stem cells against ‎radiation. Thus, curcumin may prevent sterility in men who undergo radiotherapy. ‎

کلیدواژه ها

Curcumin, Radiation therapy, Radiation protective agents, Spermatogonial stem cells, Sterility

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