Chemical Composition and Antibacterial Activity of Wild Rose (Rosa canina L.) Gall Extracts against Gram-Negative Pathogenic Bacteria

سال انتشار: 1401
محل انتشار: مجله علوم پیشرفته زیست پزشکی، دوره: 13، شماره: 1
کد COI اختصاصی: JR_JABS-13-1_002
زبان مقاله: انگلیسی
تعداد مشاهده: 246

نویسندگان

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural & Natural Resources Research Center, Khorramabad, Iran

چکیده

Background & Objectives: Treating infections due to antibiotic-resistant bacteria is a challenge and researchers are looking for new antimicrobial compounds. Galls are abnormal plant growths caused by biological agents and have active compounds. The present study was designed to examine the antibacterial effects of different extracts of wild rose gall (Rosa canina L.) against some pathogenic Gram-negative bacilli. Materials & Methods: Methanol, acetone, and aqueous extracts from galls were prepared using Soxhlet apparatus. The antibacterial activity of the extracts was determined by agar well diffusion method, and the minimum inhibitory concentration and the minimum bactericidal concentration were assessed by the microdilution method. The phytochemical composition of galls was investigated by Gas Chromatography-Mass Spectrometry (GC-MS) method. Results: The inhibition zones of ۵۰۰ mg/ml methanol extract of wild rose gall against Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae, Acinetobacter bummani, Shigella sonei, and Salmonella typhi were ۲۶.۷, ۲۵.۰, ۲۵.۷, ۲۵.۷, ۲۶.۷, ۲۳.۷ and ۱۸.۳, respectively. The antimicrobial activity of the extracts was directly related to their concentration. The extracts were active against all the pathogenic bacteria with average MICs ranging from ۱۵.۶ to ۳۱.۳ mg/ml. The methanol extract of wild rose gall showed the highest bactericidal effect on P. aeruginosa and A. bummani at ۶۲.۵ mg/ml, respectively. Moreover, oleic acid, palmitic acid, and octadecenoic acid were composed ۳۶.۶۶%, ۱۴.۴۰%, and ۱۳.۲۱% of total active compounds in wild rose gall. Conclusion: All of the wild rose gall extracts showed significant antibacterial activities against Gram-negative bacilli. The antibacterial effects may be related to the high amounts of organic acids in wild rose gall extracts

کلیدواژه ها

Anti-bacterial compound, Phytochemicals, Plant extracts, Rosa

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