Prevalence and Molecular Mapping of Multidrug-resistant Mycobacterium tuberculosis in Sokoto, North-Western Nigeria
عنوان مقاله: Prevalence and Molecular Mapping of Multidrug-resistant Mycobacterium tuberculosis in Sokoto, North-Western Nigeria
شناسه ملی مقاله: JR_JCMI-9-3_003
منتشر شده در در سال 1401
شناسه ملی مقاله: JR_JCMI-9-3_003
منتشر شده در در سال 1401
مشخصات نویسندگان مقاله:
Shuaibu Abdullahi Hudu - Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University Sokoto, ۸۴۰۲۳۲ Sokoto State, Nigeria
Abdulgafar Olayiwola Jimoh - Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria
Yahaya Mohammed - Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University Sokoto, ۸۴۰۲۳۲ Sokoto State, Nigeria
خلاصه مقاله:
Shuaibu Abdullahi Hudu - Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University Sokoto, ۸۴۰۲۳۲ Sokoto State, Nigeria
Abdulgafar Olayiwola Jimoh - Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria
Yahaya Mohammed - Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University Sokoto, ۸۴۰۲۳۲ Sokoto State, Nigeria
Background: The emergence of multidrug-resistant tuberculosis tuberculosis (MDR-TB) poses a significant
danger to Nigeria’s TB control efforts. Nigeria records ۵۷۰ ۰۰۰ new TB cases each year, and it is one of
the world’s greatest TB-burden countries as well as one of the top ten countries with MDR-TB. This study
aimed to determine the MDR prevalence, phylogenetic analysis, and molecular mapping as well as the
link between MDR prevalence and demographic data.
Methods: The study comprised ۱۰۰ TB patients recruited consecutively. The proportion method on
Lowenstein-Jensen (LJ) medium was used for drug susceptibility testing. Conventional polymerase chain
reaction (PCR) was used, and the rrs genes were amplified and sequenced. Multiple sequence alignment
techniques were used to compare the PCR product sequences to reference sequences retrieved from
GenBank.
Results: Rifampicin (RIF) resistance was found in ۲۹% (۲۲.۷۵), ethambutol resistance was found in
۲۰% (۱۵.۷۵), and isoniazid (INH) resistance was found in ۲۸% (۲۱.۷۵). Further, RIF and INH resistance
were found in ۲۰% (۱۵.۷۵) of the samples. At nucleotide position ۸۹۲, four isolates (۳۱%) contained
a G-A transition, and the most prevalent mutation found in the rrs gene was S۵۳۱L (۸۰%, ۱۲.۱۵). The
phylogenetic analysis indicated that three of the Sokoto isolates are closely linked to reference isolates
from Iran, Germany, China, and Sudan in terms of geographical relatedness.
Conclusions: The obtained data revealed that acquired resistance is a major factor in the establishment
of MDR-TB in Sokoto, which could be owing to poor adherence to medication or poor treatment of TB
patients.
کلمات کلیدی: Resistance, Tuberculosis, Multi-drug, Sokoto
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1700778/