Anti-hypercholesterolemic activity of standardized fermented Allium cepa L. var aggregatum extract: In vitro and in vivo studies
- سال انتشار: 1400
- محل انتشار: Journal of Herbmed Pharmacology، دوره: 11، شماره: 3
- کد COI اختصاصی: JR_HERM-11-3_009
- زبان مقاله: انگلیسی
- تعداد مشاهده: 57
نویسندگان
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Department of Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
۴Department of Biochemistry, Bogor Agricultural University, West Java, Indonesia
چکیده
Introduction: Allium cepa extract has been reported to have anti-hypercholesterolemic activity in rats. This study was conducted to investigate the effects of standardized fermented A. cepa L. var aggregatum extract on cholesterol levels and HMG-CoA reductase enzyme. Methods: The fermented A. cepa extract was standardized by the presence of quercetin using a validated high performance liquid chromatography (HPLC) method. The activity of the extract on HMG-CoA reductase was determined using HMG-CoA Assay kits, then measured by Nano spectrophotometry. In vivo study was conducted in hypercholesterolemic rats. The extract was administered orally at doses of ۱۰۰, ۲۰۰, and ۳۰۰ mg/kg body weight (bw) to rats for ۲۱ days and the cholesterol levels were measured every week. Results: All doses of fermented A. cepa extract and its marker compound, quercetin, ameliorated the levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) as compared to those of negative control (P< ۰.۰۵). Of all the doses, fermented A. cepa extract at the dose of ۲۰۰ mg/kg bw displayed the highest reduction on LDL-C levels. In addition, the extract at the dose of ۲۰۰ mg/kg bw showed the strongest enhancement in HDL-C levels. The fermented A. cepa extract and quercetin also inhibited the HMG-CoA reductase enzyme with inhibitory activity of ۶۱.۷۸%. Conclusion: The ethanol extract of fermented A. cepa shows anti-hypercholesterolemic activity. The strong anti-hypercholesterolemic activity of the extract might be due to the high amounts of quercetin, although other constituents may also contribute.کلیدواژه ها
Allium cepa, Fermentation, Anti-hypercholesterolemic activity Quercetin, Hypocholesterolemic activity Dyslipidemiaمقالات مرتبط جدید
- Phenolic fraction concentrates supplementation ameliorates learning and memory impairments in chronically stressed streptozotocin-diabetic rats by reducing brain tumor necrosis factor-α
- Chemical composition of Prangos ferulacea (L.) Lindl., and Prangos uloptera DC. essential oils and their antifungal activities
- Effect of ethyl acetate fraction of Costus afer on glycaemic control and essential haematological and biochemical indices of streptozotocin-induced diabetic rats
- Insulin supplemented with phenolic fraction concentrates displays anxiolytic and antidepressant-like properties with reductions of oxidative brain damage in chronically stressed diabetic rats
- Evaluation of erythrocyte viability, antioxidant capacity and antiplasmodial activity induced by alkaloid extract of Phyllanthus amarus
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.