Evaluation of the Immunogenicity of Recombinant Espb, Espc Proteins from Mycobacterium Tuberculosis and the Fusion Espc/Espb Protein in BALB/C Mice
- سال انتشار: 1401
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 11، شماره: 4
- کد COI اختصاصی: JR_RBMB-11-4_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 108
نویسندگان
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
Department of Mycobacteriology and pulmonary research, Pasteur Institute of Iran, ۶۹ Pasteur Ave., Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
چکیده
Background: Two newly identified proteins, EspB and EspC are involved in the pathogenesis of Mycobacterium tuberculosis. The objective of the present study was to evaluate the immunogenicity of recombinant EspC, EspB, and EspC/EspB fusion proteins in mice. Methods: BALB/c mice were immunized subcutaneously with recombinant EspC, EspB, and fusion EspC/EspB proteins, three times with along with Quil-A as an adjuvant. The cellular and humoral immune responses were evaluated by quantifying IFN-g, IL-۴, IgG, IgG۱, and IgG۲a antibodies against the antigens. Results: The results showed that the mice immunized with recombinant EspC, EspB, and EspC/EspB proteins did not produce IL-۴, whereas IFN-g was secreted in response to all three proteins. EspC/EspB group produced significant amounts of IFN-g in response to stimulation with all the three recombinant proteins (P< ۰.۰۰۱). In mice immunized with EspC, high levels of IFN-g were detected in response to EspC/EspB, and EspC (P< ۰.۰۰۰۱); while mice immunized with EspB produced lower levels of IFN- g in response to EspC/EspB, and EspB (P< ۰.۰۵). Conclusions: All the three recombinant proteins induced Th۱-type immune responses in mice against EspB and EspC; however, EspC/EspB protein is more desirable due to the presence of epitopes from both EspC and EspB proteins and the production of immune responses against both.کلیدواژه ها
EspB, EspC, ESX-۱, Mycobacterium tuberculosis.اطلاعات بیشتر در مورد COI
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