Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-۲۳۱ and T۴۷D as Breast Cancer Cell Lines
- سال انتشار: 1402
- محل انتشار: مجله فیزیک پزشکی ایران، دوره: 20، شماره: 2
- کد COI اختصاصی: JR_IJMP-20-2_009
- زبان مقاله: انگلیسی
- تعداد مشاهده: 166
نویسندگان
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Department of Biology, Islamshahr Branch, Islamic Azad University, Tehran, Iran
Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran, Iran
Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, IRAN
Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
چکیده
Introduction: Breast cancer is one of the most prevalent diseases around the world. Breast cancer patients treated with radiation may face Side effects as well as cancer recurrence. Some polyphenols exhibit antioxidant effects. Citrus and Orthosiphon Stamineus have source of methylated flavone called Sinensetin (SIN). In this research, we designation of Sinensetin as increasing radiation sensitivity.Material and Methods: The cytotoxic effect of Sinensetin was examined in MDA-MB-۲۳۱ and T۴۷D by MTT assay. As well as, the clonogenic ability of cells were Assessmented in the presence of Sinensetin and combination with radiation. To quantify expression alterations of apoptosis related genes, utilized Real-Time PCR method.Results: In a dose and time-dependent manner, Sinensetin decreased the viability of MDA-MB-۲۳۱ and T۴۷D. The survival fraction was decreased in cells treated with Sinensetin (SIN) prior to X-irradiation compared to cells treated with X-ray only. More ever, expression level of, Bcl-۲, STAT۳, and increased P۵۳ via treated cells with Sinensetin (SIN) and X-ray.Conclusion: Due to the results, Sinensetin (SIN) can be mentioned as a novel radiosensitizer and its effects may considered increasing apoptosis following DNA damage induced by irradiation.کلیدواژه ها
Breast Cancer X, Radiation Sinensetin Radio Sensitizer Agent Flavonoidsاطلاعات بیشتر در مورد COI
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