Investigating the frequency and antibiotic resistance pattern of Salmonellasamples isolated from dogs in Tabriz city in ۲۰۲۱

Background and objective: Salmonellosis is known as one of the common diseases between humansand animals that some species of Salmonella may also be found in dogs and cats. This issue can also bedangerous for human health, especially when it comes into contact with pets carrying the bacteria or isexposed to the environment where these animals live. Salmonella has been found in domestic dogsworldwide. However, prevalence rates vary across regions. Therefore, the present study was conducted inorder to investigate the occurrence of Salmonella in dogs in Tabriz city and identify the resistance ofstrains against different antibiotic agents and estimate the potential of pets as a source of Salmonellainfection.methods: For this purpose, rectal sampling with swab or feces was done from ۲۰۰ rural dogs andcompanion dogs referred to clinics in Tabriz in the eight-month period from May to January ۱۴۰۰. Fecalsamples from each pet were accompanied by a detailed questionnaire to record its breed, age, and clinicalconditions. Each stool sample was collected in a sampling tube, stored at ۴°C, and then transported to thelaboratory for further analysis within ۶ hours. A ۱-۵g sample was randomly taken from the dog's rectumor feces and added to ۱۰ ml of buffered peptone broth, after which the samples were shaken andincubated at ۳۷°C. Subsequently, colonies of MSRV cultures were grown on a selective xylose-lysineterritory۴ agar medium. Probable Salmonella isolates were selected from each environment, then placedin TSI agar and lysine decarboxylase agar media and incubated for ۲۴ hours at ۳۷°C for biochemicalanalysis. Isolates with typical phenotypes were confirmed to be Salmonella using serological test strips.To check antibiotic resistance, ciprofloxacin, amikacin, cefazolin, enrofloxacin, fosfomycin,danofloxacin, tetracycline, doxycycline, sultrim, erythromycin and amoxicillin discs were used.Results: In general, in the investigation of the contamination rate of rural and companion dogs, out of ۲۰۰dog collars under study, ۴۰ cases of Salmonella were detected. The results showed that ۳۰ cases ofSalmonella were isolated from ۱۵۰ rural dog collars and ۱۰ cases from ۵۰ domestic dog collars. In theexamination of antibiogram disks, the highest resistance was related to the antibiotics tetracycline (۶۴%),doxycycline (۷۰%), sultrim (۵۶%), and amoxicillin (۶۸%).Conclusion: Based on the results obtained in this study, dogs should be controlled and checked regularlydue to their close relationship with humans and because of the potential source of salmonellosis. Also,excessive use of antibiotics in the control of salmonellosis should be minimized and prevention methodsshould be taken into consideration.

کلیدواژه ها:

Antibiotic resistance ، Dog ، Salmonellosis ، Tabriz