Growth Media Optimization for Production of Alkaline Protease from Industrial Wastewater using Bacillus subtilis PTCC ۱۲۵۴

  • سال انتشار: 1402
  • محل انتشار: ماهنامه بین المللی مهندسی، دوره: 36، شماره: 3
  • کد COI اختصاصی: JR_IJE-36-3_011
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 324
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نویسندگان

Z. Jafari

Biotechnology Research Laboratory, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran

G. Najafpour Darzi

Biotechnology Research Laboratory, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran

H. Zare

Department of Chemical Engineering, Buein Zahra Technical University, Buein Zahra, Qazvin, Iran

چکیده

Alkaline proteases are widely used in industrial processes due to their high pH tolerance and thermal stability. In present work, the protease producing ability of Bacillus strains (Bacillus subtilis PTCC ۱۲۵۴, B. subtilis PTCC ۱۱۵۶ and B. subtilis PTCC ۱۷۱۵) was studied. B. subtilis PTCC ۱۲۵۴ showed the highest proteolytic activity and therefore, the strain was selected as the biological agent in the submerged fermentation. Cell growth kinetic model was investigated using Malthus and Logistic equations, which were relatively well fitted to the experimental data. The maximum specific growth rate for Malthus and Logistic models were ۰.۱۸۷ and ۰.۳۷۷ h-۱, respectively. The optimum culture conditions were defined as follows: pH ۹, temperature ۳۷°C, fermentation time ۷۲ h, agitation speed ۱۵۰ rpm and ۴% inoculum with medium contained ۱ g/l CaCl۲, ۰.۶ g/l K۲HPO۴, ۱ g/l KH۲PO۴, ۰.۲ g/l MgSO۴.۷H۲O, ۲ g/l sugarcane bagasse and ۴ g/l corn bran as carbon and nitrogen sources. A ۲۵% v/v industrial wastewater containing starchy waste was used as main substrate. Under optimum conditions, maximum alkaline protease activity of ۱۱۷.۴۳ U/ml was achieved. Also, the obtained protease was able to remove blood stain from cotton fabric and hydrolyze gelatin of X-ray film. Thus, this protease showed potential applications in detergent and photographic industries.

کلیدواژه ها

Alkaline Proteases, Bacillus subtilis, Low-cost substrate, Starchy Wastes, Kinetic Model

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