Molecular Detection of Leptospira spp. Isolated from Aborted Ovine Genital Swabs in Lorestan, Iran, ۲۰۱۹-۲۰۲۰
- سال انتشار: 1401
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 17، شماره: 1
- کد COI اختصاصی: JR_IJMM-17-1_012
- زبان مقاله: انگلیسی
- تعداد مشاهده: 224
نویسندگان
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
چکیده
Background and Aim: Leptospirosis is a zoonotic bacterial disease caused by the genus Leptospira. This disease is observed in human, domestic and wild mammals. Abortion is known as the most important side effect of Leptospira in infected sheep. Molecular tests such as PCR is considered for the early detection of disease which cannot trigger production of species-specific antibodies. This study was designed to detect Leptospira spp. as a cause of abortion in sheep using PCR in Lorestan province, Iran. Materials and Methods: In the current study a total of ۱۵۰ samples of vaginal swabs were randomly collected from a sheep that had an abortion in some areas of Lorestan province, Iran. The collected samples were accurately investigated to trace DNA of Leptosoira spp. using specific primers. Results & Conclusion: Based on results of Leptosoira spp. lipL۳۲ gene, ۲ positive samples (۱.۳%) were identified. Hence, it was concluded that there is nosignification relation between abortion rate and Leptospirosis in Lorestan province, Iran. It must be mentioned that due to the zoonotic nature of this bacterium, rapid diagnosis of this disease in livestock can extremely decrease outbreak of this disease in humans.کلیدواژه ها
Leptospirosis, Abortion, Sheep, PCR, لپتوسپیروز, سقط جنین, گوسفند, PCR, لرستاناطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.