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Evaluation of Ficolin-۳ (FCN۳) ۱۶۳۷delC (rs۲۸۳۵۷۰۹۲) Frameshift Mutation in Iranian Type ۲ Diabetic Subjects

عنوان مقاله: Evaluation of Ficolin-۳ (FCN۳) ۱۶۳۷delC (rs۲۸۳۵۷۰۹۲) Frameshift Mutation in Iranian Type ۲ Diabetic Subjects
شناسه ملی مقاله: JR_JIML-5-4_004
منتشر شده در در سال 1397
مشخصات نویسندگان مقاله:

Shaghayegh Pishkhan Dibazar - ۱. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Ahmad Zavaran Hosseini - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Fatemeh Yari - ۲. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Shirin Fateh - ۱. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mohammad Reza Deyhim - ۲. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

خلاصه مقاله:
Background and Aims: Ficolins are proteins that bind to carbohydrates, act as opsonins and play an important role in innate immunity. Polymorphism in ficolin-۳ gene (FCN۳) can lead to complement deficiency and increase the risk of some disorders such as diabetes. The aim of this study was to investigate the frequency of FCN۳+۱۶۳۷delC as a single nucleotide polymorphism in this gene in healthy and diabetic subjects of Iran. Materials and Methods: Blood was taken from ۳۶ diabetics and ۳۷ healthy subjects who had referred to the Iranian Blood Transfusion Organization. Blood sugar was analyzed using a calorimetric method. After DNA extraction using salting out method, polymerase chain reaction (PCR) was carried out and the restriction fragment length polymorphism (RFLP) method was accomplished using ApaI restriction enzyme. Consequently, the resulted fragments were evaluated using electrophoresis on ۲% L-agarose gel. Results: Evaluation of the results indicated that the heterozygote form of single-nucleotide polymorphism FCN۳+۱۶۳۷delC was seen in three samples (۸.۱%) of the studied healthy subjects and in two samples (۵.۶%) of the diabetic individuals. Besides, the homozygous form of the mutation was not seen in the studied healthy and diabetic subjects. Conclusions: Results of this study showed that FCN۳ variant of single-nucleotide polymorphism FCN۳+۱۶۳۷delC was not considered as a risk factor for type ۲ diabetes mellitus in Iranian subjects.

کلمات کلیدی:
Diabetes mellitus, FCN۳, Polymerase chain reaction, Polymorphism, فیکولین ۳, پلی مورفیسم, واکنش زنجیره ای پلیمراز

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1609609/