Distribution and Characterization of Dominant Serovars of Listeria monocytogenes Strains Isolated from Spontaneous Human Abortion in Tehran
- سال انتشار: 1397
- محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 5، شماره: 4
- کد COI اختصاصی: JR_JIML-5-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 152
نویسندگان
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Shila Medical Diagnostic Laboratory, Tehran, Iran.
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
چکیده
Background and Aims: The aim was to determine the role of dominant serovars of Listeria monocytogenes (L. monocytogenes) in spontaneous abortions, using isolation methods and polymerase change reaction (PCR). Materials and Methods: A total of ۲۵۸ samples comprising of placental tissue, vaginal swabs and blood were collected from ۱۲۳ patients with spontaneous abortion. L. monocytogenes was identified and confirmed by culture, biochemical reactions, serological tests, API system, CAMP (Christie, Atkins, Munch and Petersen) test, and hemolysis on sheep blood agar. Phosphatidyl inositol specific phospholipase C (PI-PLC) assay, followed by multiplex PCR was applied for detection of serotypes ۱/۲a and ۴b. Result: Out of ۲۵۸ samples, ۲۸ isolates of L. monocytogenes were identified by different methods. All of the isolates were confirmed by PCR. Of ۲۸ isolated strains, ۱۴(۵۰%) belonged to serovar ۱/۲a, ۱۰(۳۵.۷%) to serovar ۴b and ۴(۱۴.۳%) to other serovars. Conclusions: Based on our study, serovars ۱/۲a and ۴b are dominant serovars as causative agents of human spontaneous abortion due to L. monocytogenes in pregnant women.کلیدواژه ها
L. monocytogenes, Serovars, Spontaneous abortion, Keywords: Humans, Listeria monocytogenes, spontaneous abortion, dominant serovars, hly gene, iap geneاطلاعات بیشتر در مورد COI
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