Myeloid Cell Leukemia-۱ Gene Expression and Clinicopathological Features in Myelodysplastic Syndrome
- سال انتشار: 1397
- محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 6، شماره: 1
- کد COI اختصاصی: JR_JIML-6-1_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 139
نویسندگان
Department of Hematology, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
چکیده
Background and Aims: Myeloid cell leukemia-۱ (Mcl-۱) plays a pivotal role in the survival of hematologic and solid tumors, and is known as a substantial oncogene. Studies have demonstrated the altered expression of Mcl-۱ has been linked to malignancy development and poor prognosis. In this research, we have studied the expression of Mcl-۱ mRNA in myelodysplastic syndrome (MDS) patients and determined association with clinico-pathological factors, MDS subgroups as well as international prognostic scoring system. Materials and Methods: The relative level of Mcl-۱ was determined by real time quantitative real-time polymerase chain reaction and gene expression normalized to Glyceraldehyde-۳-phosphate dehydrogenase. Results: Results indicated amplification of mRNA encoding Mcl-۱ in ۱۰۰% of the cases. The higher level of Mcl-۱ existed in MDS patients compared with the healthy controls but there was no statistically difference of Mcl-۱ expression between these groups. Fold change in gene expression was higher in advanced stage MDS, high risk MDS, cases with > ۵% blast and LDH > ۴۰۰ to their corresponding groups. In addition, the correlation between gene expression and cytogenetic prognostic subgroups was statistically significant (p=۰.۰۴۳). Conclusions: In the present study, we showed that Mcl-۱ is expressed in MDS independent of the World Health Organization subgroup and international prognostic scoring system. Therefore, Mcl-۱ may be up-regulated already in early stages of leukemogenesis.کلیدواژه ها
Mcl-۱, Myelodysplastic syndromes, Real time PCRاطلاعات بیشتر در مورد COI
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