Association of cytotoxic T-lymphocyte-associated protein ۴ polymorphisms with recurrent pregnancy loss: A case-control study

Background: A large proportion of cases of recurrent pregnancy loss (RPL) are associated with immunological factors. Objective: This study investigated the association between single nucleotide polymorphisms of cytotoxic T-lymphocyte-associated protein (CTLA)-۴ gene in women with a history of RPL compared to healthy women. Materials and Methods: A case-control study was performed on ۲ groups consisting of ۱۲۰ healthy women with no history of abortion and at least one delivery (control) and ۱۲۰ women with a history of ۲ or more primary RPLs (case). In addition, ۵ mL of peripheral blood sample was taken from all subjects. The frequencies of CTLA-۴ rs۳۰۸۷۲۴۳ and rs۲۳۱۷۷۵ polymorphisms were assayed by restriction fragment length polymorphism polymerase chain reaction and rs۵۷۴۲۹۰۹ using the high-resolution melting real-time polymerase chain reactionmethod. Results: The mean age of the women in the control and RPL groups were ۳۰.۰۳ ± ۴.۲۳ (range ۲۱-۳۷), and ۲۸.۶۴ ± ۳.۶۱ yr (range ۲۰-۳۵), respectively. Pregnancy loss numbers ranged between ۲-۶ in women with a history of RPL, and between ۱ and ۴ in the successful pregnancy group. Statistical analysis showed a significant difference between the genotypes of GG and AG in the ۲ groups in rs۳۰۸۷۲۴۳ polymorphism (OR ۱.۰۰ for GG genotype and OR ۲.۸۷ for AG genotype, p = ۰.۰۰۴۳). No significant difference was observed in the genotype frequencies of rs۲۳۱۷۷۵ and rs۵۷۴۲۹۰۹ polymorphisms, of the ۲ groups (p = ۰.۳۷, and p = ۰.۰۹۵), respectively. Conclusion: Our findings indicated that CTLA-۴ polymorphism, rs۳۰۸۷۲۴۳, might be associated with a risk of RPL in Iranian women.

کلیدواژه ها:

Recurrent pregnancy loss ، Single nucleotide polymorphisms ، CTLA-۴ gene. ، سقط مکرر ، پلیمرفیسم تک­نوکلئوتیدی ، ژن CTLA-۴.