Aptamer-based Electrochemical Detection of Tyrosinamide Using Metal-organic Frameworks/silver Nanoparticles Modified Glassy Carbon Electrode
- سال انتشار: 1400
- محل انتشار: مجله تحقیقات شیمی تجزیه و تجزیه زیستی، دوره: 8، شماره: 3
- کد COI اختصاصی: JR_ANALCH-8-3_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 108
نویسندگان
Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Department of Chemistry, Khorramabad Branch Islamic Azad University Khorramabad, Iran
چکیده
In this report, a new aptamer-based assay was presented reporting the electrochemical aptasensing for sensing tyrosinamide (Tyr-NH۲). This strategy was relied on unbeatable conformational flexibility and specific recognition of aptamers. The tyrosinamide aptamer (Tyr-NH۲-aptamer) was immobilized onto the metal-organic frameworks/silver nanoparticles modified glassy carbon electrode and hexacyanoferrate was selected as a probe to monitor interface variations during modification of the electrode and the aptamer conformational change generated by the Tyr-NH۲ binding. Results showed that measurements by using electrochemical impedance spectroscopy had linear with the Tyr-NH۲ concentrations in range of ۰.۰۱-۰.۲۵ nM and ۰.۲۵-۱.۱۵ nM. Detection limit of this system was found to be ۲.۳ pM. This method was also used to the Tyr-NH۲ detection in serum samples successfully. Remarkable simplicity, ease of use and low-cost, make methodology as sensitive analytical system for sensing of the Tyr-NH۲ that can be miniaturized. This strategy offers some promising advantages in reliable detection of the Tyr-NH۲, which may be helpful in the routine analysis.کلیدواژه ها
Electrochemical aptasensor, Tyrosinaimde, Hexacyanoferrate, Metal-organic Frameworks, Silver nanoparticlsاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.