Hamid Reza Akbari-Hasanjani - Department of Chemistry, Faculty of Chemistry, Azad University, North Tehran Branch, Tehran, Iran
Mahmoud Reza Sohrabi - Department of Chemistry, Faculty of Chemistry, Azad University, North Tehran Branch, Tehran, Iran
Parviz Abdolmaleki - Department of Biophysics, Faculty bof Biological Sciences, Tarbiat Modares University, Tehran, Iran

The net analyte signal standard addition method was used for simultaneous spectrophotometric determination of sertraline and fluoxetine in pharmaceutical preparations. The method combines the advantages of the standard addition method with the net analyte signal concept to enable the extraction of information about an analyte from the spectra of multi-component mixtures. This method uses full spectrum realization and does not require calibration and prediction steps. Determination requires only a few measurements. The limit of detection for fluoxetine was ۰.۳۱ µg ml-۱ and for sertraline was ۰.۲۰ µg ml-۱. The root mean square error for fluoxetine was ۰.۴۵ and for sertraline was ۰.۳۹.

fluoxetine, Sertraline, antidepressant, Net analyte signal, spectrophotometric