Comparative Analyses of the Volatile Components of Citrus Aurantium L. Flowers Using Ultrasonic-Assisted Headspace SPME and Hydrodistillation Combined with GC-MS and Evaluation of their Antimicrobial Activities
- سال انتشار: 1393
- محل انتشار: مجله تحقیقات شیمی تجزیه و تجزیه زیستی، دوره: 1، شماره: 2
- کد COI اختصاصی: JR_ANALCH-1-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 290
نویسندگان
Department of Chemistry, Faculty of Sciences, Lorestan University, Khoramabad, Iran
Department of Chemistry, Faculty of Sciences, Lorestan University, Khoramabad, Iran
Microbiology Laboratory, Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Razi Street, Khoramabad ۶۸۱۹۸, Iran
Department of Environmental Health, School of Public Health, Infectious Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Department of Chemistry, Faculty of Sciences, Lorestan University, Khoramabad, Iran
چکیده
The volatile components of Citrus aurantium L. flowers were characterized by GC-MS with two different extraction techniques, hydrodistillation (HD) and ultrasonic-assisted headspace solid phase microextraction (UA-HS-SPME). In the SPME method, the volatile components of the samples, irradiated by ultrasonic radiation, were collected on a polydimethyl siloxane (PDMS) commercial fiber as well as some manually prepared nanoporous fibers from the samples headspace. To reach the better results, the extraction conditions were carefully optimized for the PDMS fiber. Under the optimized conditions (i.e. sonication time ۱۵ min, extraction time ۳۰ min and extraction temperature ۵۵ ºC), ۵۴ compounds were identified by the UA-HS-SPME-GC/MS method. The essential oil components of Citrus aurantium L. flower samples from two different regions of Iran and new and old samples from the same region were compared to one another. The major components identified for the samples with both the SPME and HD methods were linalool, linalyl acetate, limonene, β-myrcene, geranyl acetate, and neryl acetate, respectively. However, a substantial variation in the percentages of the components was identified for different samples and different extraction methods. The antimicrobial activities of the oil were also examined against six standard bacteria. There was some activity against Enterococcus fecalis, Escherichia coli, and Bacillus cereus, indicating important biological activities of the oil.کلیدواژه ها
Ultrasonic-assisted headspace SPME (UA-HS-SPME), Polydimethyl siloxane, Citrus aurantium L. flowers, Antimicrobial activityاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.