The inhibitory effects of Naringenin on proliferation and migration of C۶ glioblastoma cell line

Background and aim: Gliomas are the most prevalent type of malignant primary brain tumors with poor prognostic features. The aberrant Hedgehog-Gli pathway as highly conserved signaling has been found to be involved in the development of numerous malignancies including gliomas. Naringenin appears to have anti-cancer properties. The present research was set to study the anti-cancer effects of naringenin on C۶ cells to determine the effect of this compound on the Hh signaling pathway.Materials and method: The apoptotic and the anti-proliferative effects of naringenin against C۶ cells were measured by Annexin-V/PI dual staining assay and MTT assay, respectively. To analyze the anti-cancer effect of naringenin on the Hh signaling pathway, the protein expression levels of Gli-۱, Smo, and Sufu in C۶ cells were measured using western blotting. In addition, the effect of naringenin on the migration of C۶ cells was assessed by migration scratch assay.Results: Obtained data showed that naringenin induced higher cytotoxicity against C۶ cells than normal ۳T۳ fibroblasts with an IC۵۰ value of ۱۲۵ μg/ml. Naringenin (۱۲۵ μg/ml) also induced stronger apoptotic effects on C۶ cells than ۳T۳ cells after ۲۴ h of incubation. In addition, naringenin at a concentration of ۱۲۵ μg/ml significantly reduced the expression of Gli-۱ and Smo as well as increased the expression of Sufu at the protein level in the C۶ cell line. Furthermore, naringenin at concentrations of ۶۲ and ۱۲۵ μg/ml attenuated the migration of the C۶ cell line.Conclusion: These data indicate that naringenin may have a potential effect on the management of the proliferation and metastasis of malignant gliomas by attenuating the Hh signaling pathway.

کلیدواژه ها:

Naringenin ، Glioblastoma ، C۶ ، Hedgehog signaling pathway ، Gli-۱ ، Migration