Molecular Study of Partial Deletions of AZFc Region of the Y Chromosome in Infertile Men
- سال انتشار: 1392
- محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 20، شماره: 5
- کد COI اختصاصی: JR_JKMU-20-5_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 213
نویسندگان
Department of Genetics, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Assistant Professor, Department of Genetics, School of Sciences, University of Isfahan, Isfahan, Iran
Assistant Professor, Department of Genetics, School of Sciences, University of Isfahan, Isfahan, Iran
Associate Professor, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
چکیده
Background & Aims: The most significant cause of infertility in men is the genetic deletion in the azoospermia factor (AZF) region that is caused by the process of intra- and inter-chromosomal homologous recombination in amplicons. Homologous recombination could also result in partial deletions in AZF region. The aim of this research was to determine the association between the partial AZFc deletions and infertility. Methods: The blood samples were taken from ۱۰۰ infertile men‚ who referred to the Infertility Center of Isfahan‚ Iran. ۱۰۰ healthy matched people were also selected as the control group. The five markers of sY۱۲۰۱‚ sY۱۲۰۶‚ sY۱۱۶۱‚ sY۱۲۹۱, and sY۱۱۹۱ were applied in order to study partial deletions. Partial deletions were analyzed in AZF region using the Multiplex–STS–PCR technique. The chi-square test was conducted to check the difference between pretest and posttest. Differences were considered significant if P < ۰.۰۵. Results: ۹% of studied persons showed gr/gr deletion (in the patient group). Only one case of gr/gr deletion was observed in the control group. Five patients showed b۲/b۳ deletion. One b۲/b۳ deletion was seen in the control group. The b۲/b۴ deletion was observed in ۳ patients. In conclusion‚ partial deletions were observed in ۱۴% of the patients. The statistical analysis of the gr/gr deletion in the study indicates a meaningful difference, but b۲/b۳ deletion does not represent a meaningful difference. Conclusion: Our results suggest that gr/gr deletions are associated with spermatogenic failure, and there is no association between b۲/b۳ deletion and infertilityکلیدواژه ها
Infertility, Deleted azoospermia‚ Azoospermia factor‚ STS markerاطلاعات بیشتر در مورد COI
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