Histological Evaluation of the Fetus Lung in NMRI Mice after Exposure to Iron Oxide Nanoparticles: an in vitro Study
- سال انتشار: 1395
- محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 23، شماره: 5
- کد COI اختصاصی: JR_JKMU-23-5_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 141
نویسندگان
Student of Biology, Dept. of Biology, Science and Research, Islamic Azad University, Tehran, Iran
Assistant Professor, Dept. of Biology, Branch of Parand, Islamic Azad University, Parand, Iran
Assistant Professor, Dept. of Biology, Islamic Azad University, Science and Research, Tehran, Iran
Professor, Dept. of Biology, Science and Research, Islamic Azad University, Tehran, Iran
چکیده
Background and Aim: Iron oxide nanoparticles are used in fields related to nanotechnology including ecology, magnetic storage, imaging and medicinal purposes. Iron nanoparticles produce reactive oxygen species (Ros). These materials are able to cross the placenta. The aim of this study was to investigate toxic effect of iron oxide nanoparticles on fetal lung in mice. Methods: In this study, at day ۱۴ of pregnancy, fetal lungs were removed and transferred to the Cell culture medium. The lungs were divided into ۵ groups, including control, sham and experimental groups of ۱, ۲ and ۳ (received respectively ۱۰, ۳۰ and ۵۰ mg/kg nano iron oxide) and then they were incubated for ۲۴ hours. For histopathological evaluation, lung tissues were stained with Hematoxylin-Eosin and the results were evaluated. Results: Mean number of bronchioles and the diameter of blood vessels in exprimental groups showed no significant difference compared with control and sham groups. Mean number of blood vessels in experimental groups ۱ and ۲ showed no significant difference compared to control and sham groups, while in the experimental group ۳, mean number of blood vessels showed significant decrease compared to sham and control groups (p< ۰.۰۵). Histopathological examination of lung after exposure to iron oxide nanoparticles showed signs of cytotoxicity including vacuole degeneration and necrosis. Conclusion: The finding of this study showed that in in vitro, conditions, iron oxide nanoparticles reduce number of blood vessels and have cytotoxic effects such as vacuole degeneration and necrosis in the lung tissue of the fetus, in a dose- dependent way.These results can be grounds for in vivo studies.کلیدواژه ها
Nanoparticles, Ferric oxide, Fetus, Lung, Cytotoxicityاطلاعات بیشتر در مورد COI
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