The Relationship between the Expression of Small Heat-Shock Proteins and Suppression of OCT۴B۱
- سال انتشار: 1397
- محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 25، شماره: 2
- کد COI اختصاصی: JR_JKMU-25-2_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 103
نویسندگان
Assistant Professor of Molecular Genetics, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences and Department of Clinical Biochemistry Rafsanjan University of Medical Sciences, Rafsanjan, Iran
MSc of Molecular Genetics, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Professor of Hematology, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences and Department of Immunology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Professor of Clinical Biochemistry, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences and Department of Clinical Biochemistry Rafsanjan University of Medical Sciences, Rafsanjan, Iran
MSc of Immunology, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
MSc of Biochemistry, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
چکیده
Background: OCT۴B۱, a variant of OCT۴, is expressed in both cancer cells and tissues. This variant has a main role in the regulation of both apoptotic and stress (heat-shock proteins) pathways. The aim of this study was to investigate the effects of OCT۴B۱ silencing on the expression of small heat-shock proteins (sHSPs)in three human tumor cell lines. Methods:AGS (gastric adenocarcinoma), ۵۶۳۷ (bladder tumor) and U-۸۷MG (brain tumor) cell lines were transfected with specific OCT۴B۱ siRNA (test group) and scramble siRNA (control group), using siRNA and Lipofectamine. Real-Time PCR Array technique was applied and the fold changes were calculated using RT۲ Profiler PCR Array Data Analysis version ۳.۵. Results: It was revealed that HSPB۱, HSPB۶ and HSPE۱ were down-regulated in all three studied tumor cell lines and HSPB۲, HSPB۷ and HSPB۸ were down-regulated in two of three studied tumor cell lines (AGS and ۵۶۳۷). It was also revealed that HSPB۳ was down-regulated in ۵۶۳۷ cell line and up-regulated in AGS and U۸۷MG cell lines. Conclusion: According to the results, it may be concluded that there is a direct relationship between OCT۴B۱ and sHSPs gene family expression. Thus, suppression of OCT۴B۱ may be considered in cancer therapy/research.کلیدواژه ها
OCT۴B۱, Small HSPs, Tumor cell linesاطلاعات بیشتر در مورد COI
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