Possible role of LncRNA MEG۳-microRNA-۲۱ and endoplasmic reticulum (ER) stress proteins in the Pathogenesis of Psoriasis Vulgaris

Background: Psoriasis is a chronic inflammatory immune mediated disease arising from interaction between genetic risk variants and the environment. Maternally expressed gene۳ (MEG۳) is a long noncoding RNA (lncRNA) known for gene transcription regulation and inhibiting proliferation. MEG۳ competes with microRNA (miRNA-۲۱) influencing cell proliferation and apoptosis balance. Endoplasmic reticulum (ER) stress proteins promote cell survival via unfolded protein response (UPR) influenced by MEG۳. We aimed to detect the possible role of MEG۳, miRNA-۲۱ and ER stress proteins in pathogenesis of psoriasis vulgaris.   Methods: Human GRP۷۸, ATF۶, caspase۳ tissue levels were assayed by Enzyme Linked Immunosorbent Assay (ELISA). Assessment of long non-coding MEG۳ and miRNA ۲۱ expressions was done by quantitative real time polymerase chain reaction (qRT-PCR).   Results: Expression of MEG۳ was significantly downregulated, while miRNA-۲۱ was remarkably upregulated, ER stress proteins GRP۷۸, ATF۶, and caspase ۳ all showed low levels in homogenized psoriatic lesions when compared to normal skin. miRNA ۲۱ and MEG۳ were identified as possible diagnostic markers for psoriasis vulgaris.   Conclusions: MEG۳ is barely expressed in psoriatic lesions while miRNA-۲۱ expression is remarkably elevated but when correlated to each other there was unexpected positive correlation. MEG۳ and miRNA-۲۱ were identified as possible diagnostic markers for psoriasis. Undifferentiated psoriatic lesions have very weak UPR.

کلیدواژه ها:

ER stress proteins ، lncRNA MEG۳ ، Microrna-۲۱ ، Psoriasis.