The Small Molecule Enoxacin Prohibits The In VivoGrowth and Tumorigenicity of Esophageal Cancer Cells
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_303
- زبان مقاله: انگلیسی
- تعداد مشاهده: 181
نویسندگان
Department of Developmental Biology, Faculty of Sciences andAdvanced Technologies in Biology, University of Science and Culture,Tehran, Iran.Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell
Department of Developmental Biology, Faculty of Sciences andAdvanced Technologies in Biology, University of Science and Culture,Tehran, Iran.Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell
Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell Biology andTechnology, ACECR, Tehran, Iran
چکیده
Objective: Esophageal carcinoma (EC) is one of the mos t lethalcancers worldwide. Several cancer types, including EC, showglobal downregulation of microRNAs, i.e., non-coding RNAsinvolved in pos t-transcriptional gene regulation, to enable higherinvasiveness. Therefore, we aimed to analyze if enoxacin, amicroRNA-enhancing small molecule, could inhibit EC growth in vitro and in vivo.Materials and Methods: To inves tigate enoxacin (۱۲۴ μM) inEC cells (KYSE-۳۰ line) in vitro, MTT viability assay, Crys talViolet s taining, and Live/Dead assay were performed ۷۲ hourspos t-treatment. The migratory ability of the cells was carriedout using a wound healing assay. A sub-cutaneous mouse modelof EC was es tablished using KYSE-۳۰ and immunocompromisedmice. The cells (۴×۱۰۶) were injected into nude mice۷۲ hours after treatment with enoxacin (۱ mg/kg). Tumor tissueswere removed and fixed in ۱۰% buffered formalin. Next,paraffin-embedded specimens were cut into serial sections ands tained with hematoxylin and eosin. Data were analyzed usingGraphPad Prism and ImageJ (P value < ۰.۰۵).Results: We found that enoxacin reduced the growth and viabilityof EC cells in vitro. We also observed that enoxacin wasable to res trict the migration of the cells. Furthermore, the tumorformation ability of EC cells in mouse models was decreased inenoxacin-treated cells compared to the control groups (n = ۵).Particularly, no tumors (n = ۴) or only small tumors (n = ۱) wereobserved in treated mice, judging from his tological evaluations.Finally, the tumors in the treated groups were less aggressivethan the control group.Conclusion: Enoxacin inhibits the growth and migration of ECcells in vitro and in animal models of ECکلیدواژه ها
Carcinogenesis, Esophagus, Esophageal SquamousCell Carcinoma, miRNA Pathway, RNAiاطلاعات بیشتر در مورد COI
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