Inves tigating The Presence of Extracellular Mitochondriain The Content of The S tem Cells from HumanExfoliated Deciduous Teeth (SHED) in Normal CultureConditions
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_300
- زبان مقاله: انگلیسی
- تعداد مشاهده: 122
نویسندگان
Department of Biology, Faculty of Science and Technology, ACECR,Ins titute of Higher Education, Isfahan, Iran . Department of Animal Biotechnology, Cell Science ResearchCenter, Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
Department of Animal Biotechnology, Cell Science ResearchCenter, Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
Department of Animal Biotechnology, Cell Science ResearchCenter, Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
چکیده
Objective: Mitochondria are dynamic organelles that can enterthe extracellular space under certain conditions as signalingorganelles. Mitochondrial release from s tressed cells has beendebated for years, while the presence of mitochondria in theextracellular space of cells under normal culture conditions isunknown. S tem cells from human exfoliated deciduous teeth(SHED) and their secretome, due to their ease of access andwide therapeutic applications, have been regarded as a specificcandidate for cell therapies. Therefore, the present s tudy aimedto inves tigate the presence of extracellular vesicles containingmitochondria or naked mitochondria in a culture medium ofSHED in normal culture conditions.Materials and Methods: SHED with a density of ۴۵ ×۱۰۴ cell/cm۲ were cultured in DMEM supplemented with ۱۰% FBS. Afterthe cells reached ۹۰% confluency, conditioned media werecleared from cellular debris by centrifuging at ۴۰۰ g for ۱۰ minutes.Mitochondria isolation was performed throughout ۱۰۰۰۰g, ۱۰ minutes, at ۴ °C. The pellet was solved in PBS (free calciumand magnesium) and prepared for transmission electron microscopy(TEM) and Dynamic Light Scattering (DLS) analysis.Results: We demons trate that cells could release mitochondriaunder normal culture conditions. The presence, morphology,and size of mitochondrial-related particles were detected withTEM and DLS.Conclusion: Based on the TEM and DLS results, in normalculture conditions, mitochondria are released from the cells inthe form of naked, vesicular, and even swollen mitochondriaکلیدواژه ها
Extracellular Vesicles, Mesenchymal S tem Cells, Mitochondriaاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.