In Vitro Evaluation of A Wound Dressing Composedof Human Foreskin Fibroblas t on A Bovine Collagen Sheet
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_290
- زبان مقاله: انگلیسی
- تعداد مشاهده: 127
نویسندگان
Department of Developmental Biology, Faculty of Sciences andAdvanced Technologies in Biology, University of Science and Culture,Tehran, Iran . Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cel
Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell Biology andTechnology, ACECR, Tehran, Iran
Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell Biology andTechnology, ACECR, Tehran, Iran
Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell Biology andTechnology, ACECR, Tehran, Iran
چکیده
Objective: The skin is known as the larges t organ of the bodyand also a barrier that protects the human agains t environmentalchanges. Burns is one of the mos t common injuries that occurto the skin. In this s tudy, we used a commercial collagen sheetwound dressing material as a subs trate for seeding of humanforeskin fibroblas ts (hFF) to create a biological wound dressing.Materials and Methods: The hFF cells were provided by theRoyan S tem Cell Bank and cultured in an appropriate medium.The bovine collagen sheet (Suprasorb® C) was cut out in dimensionsof ۰.۲×.۰۲ cm۲ and inserted into the wells of a ۲۴-wellplate. The cells were loaded at the density of ۱۰۵ cells/well onthe collagen sheet. The MTS assay was performed to analyzethe viability and proliferation of the cells on the collagen sheetafter one, two, and three days of incubation.Results: The MTS assay showed significantly higher cell viability and metabolic activity in cell-laden collagen cons tructsin comparison to the control group (cell only) after one day inculture. There were no significant differences between groupsafter two- and three- days of incubation.Conclusion: Based on the results of this s tudy, it can be concludedthat the collagen sheet with hFF cells could be consideredas a biological wound dressing. However, in vivo s tudy onan animal model of burn can provide more information aboutthe effectiveness of this biological bandageکلیدواژه ها
Collagen Sheet, Human Dermal Fibroblas t, WoundDressingاطلاعات بیشتر در مورد COI
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