Nitidine Chloride Suppresses The Cell Growth andMigration of Gas tric Cancer Cells In Vitro

Objective: Gas tric cancer (GC) is one of the mos t commontypes of cancer and the leading cause of cancer-related deathworldwide. Nitidine chloride (NC), a natural benzo phenanthridinealkaloid compound, shows antitumor activity agains tvarious human cancers. However, the effect of NC on GC cellgrowth and migration has remained poorly inves tigated. Here,we aim to determine the biological effects of NC on the cell viabilityand migration of GC cells.Materials and Methods: To examine the anti-cancer propertiesof NC using the human GC cell line AGS, several analyseswere done, including MTS assay, Crys tal Violet s taining, andLive/Dead assay, together with wound healing assessment. Thecell growth was inves tigated using MTS assay, crys tal violets taining, and Live/Dead assay. Wound-healing assay was performedto inves tigate the migratory ability of GC cells.Results: Based on the results of MTS viability assays, Live/Dead analysis, and Crys tal Violet s taining, we found that NCreproducibly inhibited cell viability of AGS GC cells comparedto DMSO-treated control cells. Moreover, NC was able to significantlyres trict the migration of the GC cells. Thus, NC is apotentially effective anti-cancer agent for GC treatment.Conclusion: Our findings indicate that NC exerts s trong inhibitoryeffects on human GC cells in vitro

کلیدواژه ها:

Cancer Therapy ، Cell Survival ، Motility ، NitidineChloride ، S tomach Cancer