Bone Marrow CD۳۴ Positive Cells May Be Suitablefor Collection after Death
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_283
- زبان مقاله: انگلیسی
- تعداد مشاهده: 207
نویسندگان
Department of Anatomical Sciences, Faculty of Medicine, TabrizUniversity of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, TabrizUniversity of Medical Sciences, Tabriz, Iran
Department of Applied Cell Sciences, Faculty of Advanced MedicalSciences, Tabriz University of Medical Sciences, Tabriz, Iran
S tem Cell Research Center, Tabriz University of Medical Sciences,Tabriz, Iran
چکیده
Objective: Hematopoietic s tem cells (HSCs) which are characterizedwith CD۳۴+ phenotype, have a pivotal role in bloodcell regeneration. They are located in lowes t hypoxic areas inthe bone marrow niches. This microenvironment protects themfrom DNA damage and excessive proliferation, whereas the oxygenatedarea driving cells out of quiescent s tate into proliferation.Given the resis tance of HSCs to hypoxia, it is reasonableto imagine that they can survive for some time in the absenceof oxygen.Materials and Methods: Here, we evaluated CD۳۴, Bax, Bcl-۲, Bcl-xl, and p۵۳ genes expression after death. Moreover, wees tablished the ex-vivo development of HSCs using SCF, FLT۳,IL-۲, and IL-۱۵ cytokines in culture sys tem.Results: Our finding indicated that although the mos t of thedead person's mononuclear cells were alive and adequately expressedthe CD۳۴ on their surfaces at the firs t day of isolation,the viability and CD۳۴+/Ki-۶۷ expression declined significantlyafter culture process.Conclusion: Taken together, our finding indicated that the viabilityand CD۳۴+ expression was acceptable on day ۰ and couldbe used as a novel method for therapeutic purposesکلیدواژه ها
CD۳۴ Marker, Death Individual, HematopoieticS tem Cells (HSCs), Oxygen Tensionاطلاعات بیشتر در مورد COI
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