Inves tigating The Anticancer Activity of BiosynthesizedCobalt Oxide Nanoparticles In Vitro

  • سال انتشار: 1401
  • محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
  • کد COI اختصاصی: RROYAN23_278
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 199
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نویسندگان

F Momen Eslamiehei

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

M Moghadam Matin

Department of Biology, Faculty of Science, Ferdowsi Universityof Mashhad, Mashhad, Iran . S tem Cell and Regenerative Medicine Research Group, AcademicCenter for Education, Culture and Research (ACECR)-KhorasanRazavi, Mashhad, Iran . Novel Diagnos tics an

M Mashreghi

S tem Cell and Regenerative Medicine Research Group, AcademicCenter for Education, Culture and Research (ACECR)-KhorasanRazavi, Mashhad, Iran . Novel Diagnos tics and Therapeutics Research Group, Ins tituteof Biotechnology, Ferdowsi University of Mashhad,

چکیده

Objective: In recent decades, nanotechnology has been usedfor cancer diagnosis at early s tages, and also designing drugdelivery sys tems by targeting the nano-drug combination tocancer cells, and thus reducing the cytotoxicity to normal cells.Cobalt oxide is one of the mos t potent natural anticancer agentswhich has various applications in medicine, such as drug deliverysys tems, toxicity to cancer cells, and antibacterial properties.Cytotoxicity of cobalt oxide nanoparticles (Co۳O۴NPs)can be s tudied on various cell lines.Materials and Methods: In this s tudy, we firs t producedCo۳O۴ NPs using luminescence bacteria and then characterizedtheir properties and inves tigated their anticancer activityon colorectal cancer cells. The nanoparticles were characterizedby X-ray diffraction (XRD), transmission electron microscopy(TEM), scanning electron microscopy (SEM), and Fouriertransform-infra red spectroscopy (FTIR) analysis.Results: The XRD results illus trated the success in the synthesisof Co۳O۴NPs. The results of SEM and TEM indicatedthat the biologically synthesized cobalt oxide nanoparticles arespherical and cubic in shape with about ۷۰ nm diameter. Thesuccessful biosynthesis of cobalt oxide nanoparticles, was verifiedby the presence of amine groups as shown in FTIR analysis.The cytotoxicity s tudy exhibited a time and dose dependenteffect agains t CT۲۶ colorectal cancer cells as shown by MTTassay. The half maximal inhibitory concentration (IC۵۰) wasfound to be ۶۰ μg/ml after ۴۸ hours. The cytotoxic propertiesof Co۳O۴NPs were also s tudied on mouse embryonic fibroblas tcells (NIH۳T۳) as a normal cell line, where the results indicatedlower toxicity of the NPs compared to CT۲۶ cancerous cells.Conclusion: Based on our results, the biologically synthesizedCo۳O۴NPs were found to specifically decrease the viability ofCT۲۶ cancerous cells in vitro and thus they exhibit multifunctionalproperties.

کلیدواژه ها

Anticancer Activity, Biosynthesis Cobalt Oxide Nanoparticles,CT۲۶ Cell Line, MTT assay

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