Minicircle Sys tem as An Innovative Approach forGeneration of Functional CAR T-Cell
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_276
- زبان مقاله: انگلیسی
- تعداد مشاهده: 128
نویسندگان
Department of Animal Biotechnology, Cell Science Research Center,Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
Department of Animal Biotechnology, Cell Science Research Center,Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
Department of Animal Biotechnology, Cell Science Research Center,Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
Department of Animal Biotechnology, Cell Science Research Center,Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
چکیده
Objective: Chimeric antigen receptors (CAR) are receptors thatgives T-cells a new ability to target a specific cancer antigen. Ithas been shown that this s trategy is an efficient and safe approachfor treatment of hematological and some solid cancers.On the other hand, the minicircle as a nonviral vector has theability to high level expression of the transgene without adverseeffects compared to the viral vectors. In minicircle, prokaryoticsequences are deleted from the parental plasmid and the vectoris a smaller in size with a long duration of expression, and saferdue to the absence of bacterial sequences. These characteris ticshave made the minicircle as a promising vector for biomedicalapplications. In the present s tudy, we applied minicircle as asafe vector to generate specific and functional CAR T-cells.Materials and Methods: In this s tudy, a coding sequence ofthe third generation of CAR was cons tructed, and cloned intoa replicative minicircle. This CAR encoding minicircle (CARMC)was introduced into T-cells from peripheral blood by electroporation.Then, resulted CAR T-cells were co-cultured withthe cancer cells with the specific antigen. Functionality of CAR T-cells were tes ted in vitro. Generated.Results: The results showed that CAR T-cells produced byCAR-MC, specifically targeted the cancer cells with s trongcytotoxic effects compared to negative cells. Additionally,these CAR T-cells also exhibited the ability to secret a significantlyhigher level of inflammatory cytokines compared to thecontrol T-cells.Conclusion: Overall, the present s tudy showed that the CARMChas the capability of improving antitumor activity of theT-cells. In fact, minicircle encoding a functional CAR receptorenabled the T-cells to exclusively target and des troy themalignant cellsکلیدواژه ها
Cancer Immunotherapy, CAR T-Cell, CD۱۹, Minicircleاطلاعات بیشتر در مورد COI
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