All-Trans Retinoic Acid Enhances Cytotoxicity ofCIK Cells Agains t Hepatocellular Carcinoma
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_273
- زبان مقاله: انگلیسی
- تعداد مشاهده: 231
نویسندگان
Department of Applied Cell Sciences, Faculty of Basic Sciencesand Advanced Medical Technologies, Royan Ins titute, ACECR, Tehran,Iran . Department of Regenerative Medicine, Cell Science ResearchCenter, Royan Ins titute for S tem Cell Biology and Technolog
Department of Applied Cell Sciences, Faculty of Basic Sciencesand Advanced Medical Technologies, Royan Ins titute, ACECR, Tehran,Iran . Department of Regenerative Medicine, Cell Science ResearchCenter, Royan Ins titute for S tem Cell Biology and Technolog
Department of Biology and Anatomical Sciences, School of Medicine,Shahid Beheshti University of Medical Sciences, Tehran, Iran . Department of Tissue Engineering and Applied Cell Sciences,School of Advanced Technologies in Medicine, Shahid BeheshtiUnivers
Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cell Biology andTechnology, ACECR, Tehran, Iran
Department of Regenerative Medicine, Cell Science ResearchCenter, Royan Ins titute for S tem Cell Biology and Technology,ACECR, Tehran, Iran . Department of S tem Cells and Developmental Biology, Cell ScienceResearch Center, Royan Ins titute for S tem Cel
چکیده
Objective: To determine the increased sensitivity of pretreatedhepatocellular carcinoma cells with all-trans retinoic acid(ATRA) to enhance the cytotoxicity of cytokine-induced killercells (CIKs), we evaluated their effects, alone and in combination,on HepG۲ cell line.Materials and Methods: ATRA is currently used as a potentialchemo-preventive agent because of its anti-proliferative, proapoptoticand antioxidant properties. This s tudy inves tigatedthe effects of ATRA at different concentrations on the proliferationand the expression of MHC class I chain-related protein Aand B (MICA and MICB) on HepG۲ cell line. Moreover, theanti-tumor activity of CIK cells on the pretreated HCC cell linewith ATRA analyzed as well. Peripheral blood mononuclearcells (PBMC) from healthy donor were incubated in vitro andinduced into CIK cells in the presence of various cytokines. Thephenotype, characterization and cytotoxicity of CIK cells wereidentified by flow cytometry analysis. The effect of ATRA onthe proliferation and expression of MICA/B were examined byMTS assay and quantitative RT-PCR, respectively.Results: ATRA significantly inhibited the cell growth and increasedthe expression of MICA/B on HepG۲ cells in a dosedependentmanner. In addition, CIK cells could eradicatedcancerous cells through direct interaction between NKG۲D receptorand MICA/B ligand, which are expressed on the surfaceof CIK cells and on the surface of HCC cell lines, respectively.The combined application of two approaches can synergis ticallyboos t cytotoxicity of them through upregulated expression ofMICA and MICB on the HepG۲ after ATRA treatment.Conclusion: These results sugges t that combination of ATRAand CIK could be a potentially novel treatment protocol forhepatocellular carcinoma.کلیدواژه ها
All-Trans Retinoic Acid, Cytokine-Induced KillerCells, Hepatocellular Carcinoma, Immunotherapyاطلاعات بیشتر در مورد COI
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