In A Rat Model of Cerebellar Ataxia, Human OlfactoryEpithelium-Derived S tem Cells Improve His topathologicalImpairments

Objective: Cell replacement therapy (CRT) is one of the mos teffective treatments for symptoms of neurodegenerative diseasesincluding cerebellar ataxia (CA). Due to their particularqualities, such as immunomodulatory activities and easeof access compared to other types of mesenchymal s tem cells(MSCs), human olfactory epithelium mesenchymal s tem cells(OE-MSCs) have been identified as a prospective candidate forCRT. As a result, the main purpose of our research was to seehow OE-MSC transplantation affected behavioral, s tructural,and his tological defects in a rat model of CA.Materials and Methods: OE-MSCs were extracted from individuals'nasal cavities after they gave their informed consent.The positive expression of CD۷۳, CD۹۰, and CD۱۰۵ as MSCmarkers, as well as nes tin and vimentin as primitive neuroectodermals tem cell markers, were used to identify OE-MSCs. Therats were then randomly assigned to one of the three groups:control, ۳-acetylpyridine (۳-AP) treated, or ۳-AP+ cell. Therats were given an intraperitoneal dose of ۳-AP (۷۵ mg/kg) inboth experimental groups, followed by the implantation of OEMSCsinto the ۳- AP+ cell group's cerebellum. The effect ofengrafted OE-MSCs on motor coordination and performance,as well as biochemical, immunohis tochemical, and s tereologicalchanges in the cerebellum of CA-prone rats, was s tudied.Results: The delivery of ۳-AP reduced the concentration ofGSH in the cerebellum, according to our findings. The morphologicalproperties of the cerebellar Golgi cells were similarly affectedby ۳-AP injection. OEMSC transplantation, on the otherhand, enhanced motor coordination in CA. Furthermore, when۳-AP was adminis tered, OEMSCs inhibited the caspase-۳ expressionand microglia proliferation in the cerebellum. Finally,Purkinje cells were protected from ۳-AP toxicity by OEMSCtransplantation.Conclusion: In conclusion, the current s tudy found that OEMSCshave significant benefits in the CA animal model.

کلیدواژه ها:

Caspase-۳ ، Cerebellar Ataxia ، Olfactory S tem Cell