Proliferation of Spermatogonial S tem Cells Culturedin Direct and Indirect Contact of Human Amniotic MesenchymalS tem Cells

Objective: Spermatogenesis is influenced by chemotherapyand radiography in cancerous patients especially in childrenwho have recovered. This s tudy aimed to compare the effectof direct and indirect contact of human amniotic mesenchymals tem cells (hAMSCs) on the proliferation of mouse spermatogonials tem cells (SSCs).Materials and Methods: HAMSCs were extracted fromplacental tissue and co-cultured with SSCs of ۳ to ۶-day-oldmice. SSCs were examined in three experimental groups: control,condition media and insert groups in indirect contact withhAMSCs for two weeks. The quantitative real-time polymerasechain reaction (qRT-PCR) was performed to assess proliferationgenes expression. Plzf marker was assessed by Immunohistochemis try (IHC) and C-kit, and PLZF marker was evaluatedby flow cytometry.Results: Flow cytometric analysis confirmed that the Plzf-positivecells reached ۲ fold at the end of the ۱۴th day of culturein the media group (۷۶.۴۷, P value ≤۰.۰۵). Plzf gene expressionshowed a significant increase in the media group (۱۸۸.۱ ± ۶۵,P value ≤۰.۰۵). The C-kit gene showed a significant decreasebetween both media and insert groups compared to the controlgroup. Imunocytochemis try (ICC) analysis showed a significantdifference in Plzf and C-kit markers in all groups comparedto the control.Conclusion: We were able to introduce hAMSC as a new feedercell source for increasing the proliferation of SSCs, whichcan aid males with infertility.

کلیدواژه ها:

Amniotic Mesenchymal S tem Cells ، Placenta ، Proliferation ، Spermatogenesis